Silkworm cocoons were supplied by the State Key Laboratory of Silkworm Genome Biology, Southwest University (Beibei, Chongqing, China). Dopamine hydrochloride and silver nitrate (AgNO3, AR, 99.99%) were purchased from Aladdin Corp. (Shanghai, China). Tris(hydroxymethyl)aminomethane (Tris) and hydrochloric acid (HCl) were purchased from Sangon Biotech (Shanghai, China). MiliQ water made by a MilliQ water purification system (Millipore, Billerica, MA, USA) was used in the experiments.
Tris hydroxymethyl aminomethane tris
Manufactured by Sangon
Sourced in China
Tris(hydroxymethyl)aminomethane (Tris) is a chemical compound commonly used as a buffer in biochemical and molecular biology applications. It serves as a pH regulator, maintaining a stable pH environment for various biological processes. Tris is a water-soluble, crystalline solid with the chemical formula C₄H₁₁NO₃.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Milli q water purification system, by Merck Group (2 mentions)
Epicatechin, by Chengdu Biopurify Phytochemicals (2 mentions)
Apigenin, by Chengdu Biopurify Phytochemicals (2 mentions)
Catechin, by Chengdu Biopurify Phytochemicals (2 mentions)
Hydrochloric acid (hcl), by Sangon (2 mentions)
Hydrochloric acid (hcl), by Chron Chemicals (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Live dead baclight bacterial viability kit, by Thermo Fisher Scientific (1 mentions)
S aureus, by China Center of Industrial Culture Collection (1 mentions)
Agarose, by Biowest (1 mentions)
Hypoxanthine, by Yuanye Bio-Technology (1 mentions)
Quercetin, by Chengdu Biopurify Phytochemicals (1 mentions)
Sodium chloride (nacl), by Chron Chemicals (1 mentions)
Epigallocatechin, by Chengdu Biopurify Phytochemicals (1 mentions)
Epigallocatechin gallate (egcg), by Chengdu Biopurify Phytochemicals (1 mentions)
Epicatechin gallate, by Chengdu Biopurify Phytochemicals (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Live dead cell viability kit, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
8 protocols using tris hydroxymethyl aminomethane tris
1
Silkworm Cocoon-Derived Silver Nanoparticles
2
Silkworm-derived antimicrobial materials
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Silkworm cocoons were kindly provided by the State Key Laboratory of Silkworm Genome Biology, Southwest University (Beibei, Chongqing, China). Silver nitrate (AgNO3), ZnO, and dopamine hydrochloride were from Aladdin (Shanghai, China). Agarose was provided by Biowest (Shanghai, China). Tris (hydroxymethyl) aminomethane (Tris) was from Sangon Biotech (Shanghai, China). E. coli (CICC 10389) and S. aureus (CICC 21600) were from the China Center of Industrial Culture Collection. LIVE/DEAD BacLight bacterial viability kit (L34856) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals were of analytical grade and used directly.
3
Polyvinylidene Fluoride Fiber-Based Assay
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The polyvinylidene fluoride fiber, MOF-1b, with an inner diameter of 0.5 mm and a pore size of 0.1 μm, was purchased from Tianjin Motianmo Engineering (Tianjin, China). XOD and hypoxanthine were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Allopurinol was purchased from Shanghai Macklin Biotechnology Co., Ltd. Tris(hydroxymethyl) aminomethane (Tris) was purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Dopamine was purchased from Aladdin Chemistry Co., Ltd. (Shanghai, China). Epicatechin, Epicatechin gallate, epigallocatechin, epigallocatechin gallate, quercetin, catechin, apigenin and puerarin were all purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China). HPLC-grade methanol, acetonitrile and formic acid were obtained from Beijing InnoChem Science & Technology (Beijing, China). Water used for all the experiments was purified by a water purification system (ATSelem 1820A, Antesheng Environmental Protection Equipment, Chongqing, China). Unless specified otherwise, all the other chemicals and solvents, such as sodium chloride (NaCl) and hydrochloric acid (HCl), were all of analytical grade and purchased from Chengdu Chron Chemicals Co., Ltd. (Chengdu, China).
4
Silkworm-Derived Sericin Nanoparticles
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The strain of silkworm we used in this work was a commercial silkworm strain 872. Silkworms were reared in our laboratory with fresh mulberry leaves at 25 °C and 75% relative humidity under 12 h photoperiod. Fresh silkworm cocoons were collected for sericin preparation. Dopamine hydrochloride and silver nitrate (AgNO3) were purchased from Aladdin (Shanghai, China). Hydrochloric acid and Tris (hydroxymethyl) aminomethane (Tris) were from Sangon Biotech (Shanghai, China). Ultrapure water made by a MilliQ water purification system (Millipore, Billerica, MA, USA) was used in the experiment. The Cell counting kit-8 (CCK-8) used in the experiment was bought from Beyotime (Beijing, China). LIVE/DEAD cell viability kit was bought from Thermo Fisher Scientific (Waltham, MA, USA). NIH3T3 (mouse embryonic fibroblast) cell lines were received from the China Infrastructure of Cell Line Resources. The Dulbecco’s modified Eagle’s medium (DMEM), Penicillin/Streptomycin, Fetal Bovine Serum (FBS), and Trypsin-EDTA were bought from Gibco BRL (Gaithersburg, MD, USA).
5
Fluorescent Nanomedicine Synthesis
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Doxorubicin hydrochloride, dopamine hydrochloride and Cy5 were purchased from Aladdin (Shanghai, China). mPEG-SH, 5000 Da was purchased from Ponsure. Tris-(hydroxymethyl) aminomethane (tris) was obtained from Sangon Biotech (Shanghai, China). Iso-propyl alcohol (IPA, HPLC grade) was purchased from Sinopharm Chemical Reagent. RPMI1640 was purchased from Gibco Life Technologies. Unless otherwise stated, other reagents were of analytical grade.
6
Ginsenoside Re Isolation and Characterization
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Ginsenoside Re (>98%) was obtained from the natural pharmaceutical analysis laboratory (College of Chemistry, Jilin University) and identified with the standard by HPLC method.44 (link) Anhydrous citric acid (CA, >99.5%; Aladdin Co., Shanghai, P. R. China) and ethylenediamine (EDA, >99.5%, Analytical grade; Dow Chemical Company, Midland, MI, USA) were also used in this experiment. Other chemicals including quinine sulfate, sodium chloride, sodium dodecyl sulfate, tris(hydroxymethyl) aminomethane (Tris) and ammonium persulfate were purchased from Sangon Biotech Co. (Shanghai, P. R. China). HPLC grade solvents such as methanol and acetonitrile were bought from Sigma-Aldrich Co. Ltd (St Louis, MO, USA).
DMEM, FBS, and antibiotic–antimycotic were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Trypsin with 0.02% EDTA was purchased from Biosharp Company (Beijing, P. R. China). Ultrapure water (18.20 MU/cm) from a Milli-Q ultrapure system was used in this study. PBS (1×, 1 L, pH 7.4) which was equilibrated at 37°C for 30 minutes beforehand was used in this experiment.
DMEM, FBS, and antibiotic–antimycotic were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Trypsin with 0.02% EDTA was purchased from Biosharp Company (Beijing, P. R. China). Ultrapure water (18.20 MU/cm) from a Milli-Q ultrapure system was used in this study. PBS (1×, 1 L, pH 7.4) which was equilibrated at 37°C for 30 minutes beforehand was used in this experiment.
7
Phytochemical Compound Identification Protocol
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Bovine thrombin was obtained from Sigma-Aldrich (Shanghai) Trading Co., Ltd. (Shanghai, China). p-Hydroxybenzoic acid, vanillic acid, naringenin, apigenin, baicalein, catechin, and epicatechin were purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China). Protocatechuic acid and dihydroquercetin were purchased from Push Bio-Technology Co., Ltd. (Chengdu, China). Gallic acid was the product of Aladdin Reagent Co., Ltd. (Shanghai, China). Tris(hydroxymethyl)aminomethane (Tris) was obtained from Sangon Biotech (Shanghai) Co., Ltd. (Shanghai, China). HCl and acetone were analytical grade reagents and purchased from Chengdu Kelong Chemical Reagent Factory (Chengdu, China).
8
PMMA-Based Immunoassay Development
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Polymethyl methacrylate (PMMA) sheets with different thickness were purchased from XinTao Acrylic (ShenZhen, China). Double-sided adhesive tape with a thickness of 60 μm was purchased from Wenhao (Suzhou, China). Glutaraldehyde (GA, 50% in water) was purchased from Aladdin Reagent (Shanghai, China). Poly(ethylenimine) (PEI, Mw = 70000 g mol -1 , 50 wt% aqueous solution), human immunoglobulin (IgG) and protein A from Staphylococcus aureus (SPA) were purchased from Sigma-Aldrich (MT, USA). Human AFP recombinant protein was purchased from ARP Inc. (USA). Goat anti-Chicken IgY (IgG) H&L (Alexa Fluor ® 680) and Rabbit antichicken IgG were purchased from Abcam (England). Goat antimouse IgG (IRDye ® 680) was purchased from Li-Cor (NE, USA). Rabbit anti-human AFP antibody was purchased from Abcam (England). Albumin bovine V (BSA) and human serum albumin (HSA) were purchased from Solarbio (Beijing, China). Mouse anti-human AFP antibody (C3) (Alexa Fluor ® 700) was purchased from Novusbio (USA). Fetal bovine serum (FBS) was purchased from Lonsa (South America). Human fibrinogen (HF), mouse IgG (H+L), chicken IgY (IgG) (H+L), ready-touse PBS powder and tris(hydroxymethyl)aminomethane (Tris) were purchased from Sangon Biotech (Shanghai, China).
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