Log In Sign up
The largest database of trusted experimental protocols

Quantigene 2.0 probes against tie2 and gapdh

Manufactured by Thermo Fisher Scientific
Visit Supplier

The QuantiGene 2.0 probes target the Tie2 and GAPDH genes. The probes are designed for use in gene expression analysis applications.

Automatically generated - may contain errors

Lab products found in correlation

Infinite 200 pro plate reader, by Tecan (2 mentions) Proteinase k, by Illumina (2 mentions) Tissue and cell lysis buffer, by Illumina (2 mentions) Geno grinder 2010, by SPEX (2 mentions) Quantigene 2.0 luminescent based branched dna bdna assay kit, by Thermo Fisher Scientific (2 mentions)

2 protocols using quantigene 2.0 probes against tie2 and gapdh

1

Quantifying Gene Silencing in Femoral Bone Marrow

Check if the same lab product or an alternative is used in the 5 most similar protocols
For gene silencing in whole femoral bone marrow, mice were sacrificed by CO2 asphyxiation, and femurs were harvested and immediately snap-frozen in liquid nitrogen. Frozen tissues were pulverized to form a powder using a SPEX 2010 Geno/Grinder (SPEX SamplePrep). Tissue lysates were prepared in Tissue and Cell Lysis Buffer (Epicentre) supplemented with 0.5 mg/mL Proteinase K (Epicentre). Tissue samples were mixed at 1400 RPM for 2 h at 65°C and centrifuged at 16,000 RCF to remove bone debris. mRNA levels in the supernatant were quantified using the QuantiGene 2.0 luminescent-based branched DNA (bDNA) assay kit and the QuantiGene 2.0 probes against Tie2 and GAPDH (Thermo Fisher Scientific) according to the manufacturer’s protocol. Luminescent signals were measured using a Tecan Infinite 200 PRO plate reader (Tecan). Standard curves for femur tissues and each target gene were constructed using samples from untreated mice to ensure optimal dilutions for assay samples that avoid luminescent signal saturation. Targeted gene silencing in treated mice was quantified by calculating the ratio of target gene luminescence to Gapdh gene luminescence, with all values normalized to target gene:Gapdh gene ratios from control mice.
Krohn-Grimberghe M., Mitchell M.J., Schloss M.J., Khan O.F., Courties G., Guimaraes P.P., Rohde D., Cremer S., Kowalski P.S., Sun Y., Tan M., Webster J., Wang K., Iwamoto Y., Schmidt S.P., Wojtkiewicz G.R., Nayar R., Frodermann V., Hulsmans M., Chung A., Hoyer F.F., Swirski F.K., Langer R., Anderson D.G, & Nahrendorf M. (2020). Nanoparticle-encapsulated siRNAs for gene silencing in the haematopoietic stem-cell niche. Nature biomedical engineering, 4(11), 1076-1089.
+ Open protocol
+ Expand
2

Quantifying Gene Silencing in Femoral Bone Marrow

Check if the same lab product or an alternative is used in the 5 most similar protocols
For gene silencing in whole femoral bone marrow, mice were sacrificed by CO2 asphyxiation, and femurs were harvested and immediately snap-frozen in liquid nitrogen. Frozen tissues were pulverized to form a powder using a SPEX 2010 Geno/Grinder (SPEX SamplePrep). Tissue lysates were prepared in Tissue and Cell Lysis Buffer (Epicentre) supplemented with 0.5 mg/mL Proteinase K (Epicentre). Tissue samples were mixed at 1400 RPM for 2 h at 65°C and centrifuged at 16,000 RCF to remove bone debris. mRNA levels in the supernatant were quantified using the QuantiGene 2.0 luminescent-based branched DNA (bDNA) assay kit and the QuantiGene 2.0 probes against Tie2 and GAPDH (Thermo Fisher Scientific) according to the manufacturer’s protocol. Luminescent signals were measured using a Tecan Infinite 200 PRO plate reader (Tecan). Standard curves for femur tissues and each target gene were constructed using samples from untreated mice to ensure optimal dilutions for assay samples that avoid luminescent signal saturation. Targeted gene silencing in treated mice was quantified by calculating the ratio of target gene luminescence to Gapdh gene luminescence, with all values normalized to target gene:Gapdh gene ratios from control mice.
Krohn-Grimberghe M., Mitchell M.J., Schloss M.J., Khan O.F., Courties G., Guimaraes P.P., Rohde D., Cremer S., Kowalski P.S., Sun Y., Tan M., Webster J., Wang K., Iwamoto Y., Schmidt S.P., Wojtkiewicz G.R., Nayar R., Frodermann V., Hulsmans M., Chung A., Hoyer F.F., Swirski F.K., Langer R., Anderson D.G, & Nahrendorf M. (2020). Nanoparticle-encapsulated siRNAs for gene silencing in the haematopoietic stem-cell niche. Nature biomedical engineering, 4(11), 1076-1089.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!