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Tpplabtech mosquito hts

Manufactured by Formulatrix
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The TPPLabtech Mosquito HTS is a high-throughput screening (HTS) liquid handling system. It is designed for automated and precise sample preparation and compound dispensing in a 96-, 384-, or 1536-well plate format.

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Lab products found in correlation

200 cycle kit, by Illumina (2 mentions) Mantis, by Formulatrix (2 mentions) Nextera xt kit, by Illumina (2 mentions) Nextseq, by Illumina (2 mentions)

Spelling variants of this product

Mosquito HTS (3 citations)

2 protocols using tpplabtech mosquito hts

1

Single-Cell RNA-seq Using Smart-seq-2

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysis, and cDNA synthesis was performed using the Smart-seq-2 protocol as described previously 68 ,69 , with some modifications. After cDNA amplification (23 cycles), cDNA concentrations were determined via capillary electrophoresis and cells were cherry-picked to improve quality and cost of sequencing. Cell selection was done through custom scripts and simultaneously normalizes cDNA concentrations to ~0.2 ng/uL per sample, using the TPPLabtech Mosquito HTS and Mantis (Formulatrix) robotic platforms. Libraries were prepared using the Illumina Nextera XT kits following the manufacturer’s instructions. Libraries were then sequenced on the Nextseq (Illumina) using 2 × 75bp paired-end reads and 2 × 8bp index reads with a 200 cycle kit (Illumina, 20012861) and pooled using the Mosquito liquid handler. Library quality was assessed via capillary electrophoresis on a Fragment Analyzer (AATI) and quantified by qPCR. Samples were sequenced at an average of 700,000 reads per cell.
Yousef H., Czupalla C.J., Lee D., Chen M.B., Burke A.N., Zera K.A., Zandstra J., Berber E., Lehallier B., Mathur V., Nair R.V., Bonanno L.N., Yang A.C., Peterson T., Hadeiba H., Merkel T., Körbelin J., Schwaninger M., Buckwalter M.S., Quake S.R., Butcher E.C, & Wyss-Coray T. (2019). Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nature medicine, 25(6), 988-1000.
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2

Single-Cell RNA-seq Using Smart-seq-2

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysis, and cDNA synthesis was performed using the Smart-seq-2 protocol as described previously 68 ,69 , with some modifications. After cDNA amplification (23 cycles), cDNA concentrations were determined via capillary electrophoresis and cells were cherry-picked to improve quality and cost of sequencing. Cell selection was done through custom scripts and simultaneously normalizes cDNA concentrations to ~0.2 ng/uL per sample, using the TPPLabtech Mosquito HTS and Mantis (Formulatrix) robotic platforms. Libraries were prepared using the Illumina Nextera XT kits following the manufacturer’s instructions. Libraries were then sequenced on the Nextseq (Illumina) using 2 × 75bp paired-end reads and 2 × 8bp index reads with a 200 cycle kit (Illumina, 20012861) and pooled using the Mosquito liquid handler. Library quality was assessed via capillary electrophoresis on a Fragment Analyzer (AATI) and quantified by qPCR. Samples were sequenced at an average of 700,000 reads per cell.
Yousef H., Czupalla C.J., Lee D., Chen M.B., Burke A.N., Zera K.A., Zandstra J., Berber E., Lehallier B., Mathur V., Nair R.V., Bonanno L.N., Yang A.C., Peterson T., Hadeiba H., Merkel T., Körbelin J., Schwaninger M., Buckwalter M.S., Quake S.R., Butcher E.C, & Wyss-Coray T. (2019). Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nature medicine, 25(6), 988-1000.
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