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Plenti 4.1 ex

Manufactured by Addgene
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The PLenti 4.1 Ex is a lentiviral expression vector that can be used to express genes of interest in target cells. The vector contains a multiple cloning site for insertion of the gene of interest and a Puromycin resistance gene for selection of transduced cells.

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Lab products found in correlation

Lipofectamine 2000 3000, by Thermo Fisher Scientific (2 mentions) Rmm4532 eg11863, by GE Healthcare (2 mentions) Rmm4532 eg214133, by GE Healthcare (2 mentions) Rmm4532 eg194388, by GE Healthcare (2 mentions) Plenti 4.1 ex for expression, by Addgene (2 mentions) Pcag rfp mir scrint, by Addgene (2 mentions)

2 protocols using plenti 4.1 ex

1

Lentiviral Transduction for shRNA/miRNA Expression

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Lentiviral particles for shRNA delivery was obtained as previously described7 (link) from the filtered growth media of 2x106 HEK293T transfected with 3 µg psPAX, 1 µg pVSVG and 4 µg of the plasmid of interest using Lipofectamine 2000/3000 (Life Technology). 1x105 cells of the indicated genotype were then plated onto 6-well plates and infected with the viral supernatant in the presence of 4 µg x mL-1 polybrene. After two days, the medium was replaced with selection medium containing 1 µg x mL-1 puromycin. pGIPZ vectors for shRNA against mouse HIF1β (RMM4532-EG11863), Tet2 (RMM4532-EG214133), and Tet3 (RMM4532-EG194388) were purchased from GE Healthcare UK. pLenti 4.1 Ex for expression of microRNAs was purchased from Addgene (Plasmid #35533 and #35534). pLenti 4.1 Ex scrambled vector was generated cloning a scrambled DNA sequence taken from a commercially available vector (pCAG-RFP-miR-Scrint Addgene no. 198252) into the empty backbone.
Sciacovelli M., Gonçalves E., Isaac Johnson T., Roberto Zecchini V., da Costa A.S., Gaude E., Vercauteren Drubbel A., Julian Theobald S., Abbo S., Tran M., Rajeeve V., Cardaci S., Foster S., Yun H., Cutillas P., Warren A., Gnanapragasam V., Gottlieb E., Franze K., Huntly B., Richard Maher E., Henry Maxwell P., Saez-Rodriguez J, & Frezza C. (2016). Fumarate is an epigenetic modifier that elicits epithelial-to-mesenchymal transition. Nature, 537(7621), 544-547.
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2

Lentiviral Transduction for shRNA/miRNA Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lentiviral particles for shRNA delivery was obtained as previously described7 (link) from the filtered growth media of 2x106 HEK293T transfected with 3 µg psPAX, 1 µg pVSVG and 4 µg of the plasmid of interest using Lipofectamine 2000/3000 (Life Technology). 1x105 cells of the indicated genotype were then plated onto 6-well plates and infected with the viral supernatant in the presence of 4 µg x mL-1 polybrene. After two days, the medium was replaced with selection medium containing 1 µg x mL-1 puromycin. pGIPZ vectors for shRNA against mouse HIF1β (RMM4532-EG11863), Tet2 (RMM4532-EG214133), and Tet3 (RMM4532-EG194388) were purchased from GE Healthcare UK. pLenti 4.1 Ex for expression of microRNAs was purchased from Addgene (Plasmid #35533 and #35534). pLenti 4.1 Ex scrambled vector was generated cloning a scrambled DNA sequence taken from a commercially available vector (pCAG-RFP-miR-Scrint Addgene no. 198252) into the empty backbone.
Sciacovelli M., Gonçalves E., Isaac Johnson T., Roberto Zecchini V., da Costa A.S., Gaude E., Vercauteren Drubbel A., Julian Theobald S., Abbo S., Tran M., Rajeeve V., Cardaci S., Foster S., Yun H., Cutillas P., Warren A., Gnanapragasam V., Gottlieb E., Franze K., Huntly B., Richard Maher E., Henry Maxwell P., Saez-Rodriguez J, & Frezza C. (2016). Fumarate is an epigenetic modifier that elicits epithelial-to-mesenchymal transition. Nature, 537(7621), 544-547.
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