Four-micron-thick paraffin-embedded sections were collected and stained for H&E and were incubated overnight with the following antibodies: anti-c-Myc (Abcam, Cambridge, United Kingdom; ab32072), anti-KI-67 (Abcam, ab15580), anti-G6PD (Abcam, ab87230), anti-GLUL (Sigma-Aldrich G2781), anti-GLS (Abcam, ab 262716), and anti-CASP3 (Cell Signaling Technology, Danvers, MA; 9664L), and anti-CDKN1A (Abcam, ab107099). The secondary antibodies anti-rabbit Dako EnVision+ System Labelled Polymer-HRP (Dako, Carpinteria, CA) or goat anti-rabbit Biotinylated IgG Antibody (H+L) (Vector Laboratories, Newark, CA; BA-1000) were used. Peroxidase binding sites were detected by Vector NovaRED Peroxidase (HRP) Substrate Kit (Vector Laboratories).
Anti glul
Manufactured by Merck Group
Sourced in United Kingdom
Anti-GLUL is a laboratory reagent used for the detection and quantification of the glutamine synthetase (GLUL) enzyme. It functions as an antibody that specifically binds to and identifies the GLUL protein in biological samples, enabling researchers to study its expression and activity.
Automatically generated - may contain errors
Lab products found in correlation
Anti casp3, by Cell Signaling Technology (1 mentions)
Vector novared peroxidase hrp substrate kit, by Vector Laboratories (1 mentions)
Anti g6pd, by Abcam (1 mentions)
Anti gls, by Abcam (1 mentions)
Anti cdkn1a, by Abcam (1 mentions)
Dako envision system hrp labelled polymer, by Agilent Technologies (1 mentions)
Anti ki67, by Abcam (1 mentions)
Anti c myc, by Abcam (1 mentions)
Ecl plus detection kit, by GE Healthcare (1 mentions)
Anti odc, by Santa Cruz Biotechnology (1 mentions)
Imagequant las 4000 mini, by GE Healthcare (1 mentions)
Anti beclin 1, by Cell Signaling Technology (1 mentions)
Anti gss, by Santa Cruz Biotechnology (1 mentions)
Anti idh1, by Santa Cruz Biotechnology (1 mentions)
Anti fasn, by Cell Signaling Technology (1 mentions)
Anti lc3b, by Cell Signaling Technology (1 mentions)
2 protocols using anti glul
1
Immunohistochemical Analysis of Metabolic Proteins
2
Protein Expression Profiling of Aged Diaphragm
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein extracts (50 μg) from diaphragm muscles of a pool of 6-, 12- and 24-month-old wild-type and Col6a1−/− mice were loaded in duplicate and resolved on 6%, 10% and 12% polyacrylamide gels, according to protein molecular weight. Blots were then incubated with the following antibodies: anti-fructose- 1,6-bisphosphatase (anti-FBP1; Novus biologicals NBP1-95662, 1:500), anti-Hexokinase (anti-HK; Cell Signaling Technology #2024, 1:500), anti-GLUL (Sigma Aldrich, G2781, 1:500), anti-ODC (Santa Cruz Biotechnology sc-34181, 1:1000), anti-GSS (Santa Cruz Biotechnology sc-28966, 1:500), anti-IDH1 (Santa Cruz Biotechnology sc-49996, 1:500), anti-FASN (Cell Signaling Technology #3189, 1:500), anti-Beclin 1 (Cell Signaling Technology #3738, 1:1000) and anti-LC3B (Cell Signaling Technology #2775 1:1000). After washing, membranes were incubated with anti-rabbit (GE Healthcare), anti-mouse or anti-goat (Santa Cruz Biotechnology) secondary antibodies conjugated with horseradish peroxidase. Signals were visualized by chemiluminescence using the ECL Plus detection kit and the ImageQuant LAS 4000 mini (GE Healthcare) digital imaging system.
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