5975c agilent triple axis ms detector

The GC-MS system used comprised of a 7000B Agilent GC triple-quadrupole and a 5975C Agilent triple-axis MS detector (Agilent Technologies, CA, USA). A MPS2XL GC-MS autosampler (Gerstal Technologies, Mülheim, Germany) was set to select samples for analysis in a randomised order. MeOX and TMS derivatised samples were injected onto the GC column using a hot needle technique. The injection was operated in splitless (1 µl sample) and split (0.20 µl sample) modes to avoid overloaded chromatogram peaks. The instrumentation conditions and data handling procedures (including mass spectra and peak verification processes) were as previously described [19] . Overloaded peaks (lactate, glucose, mannose, sucrose, fructose, urea and cholesterol) were analysed separately from the split mode. Muscle metabolite concentrations (expressed as arbitrary units (AU)) for each metabolite detected in each sample were normalised to the ISTD ( 13 C 6 -Sorbitol) and to muscle sample wet weight.

The GC-MS system used comprised of a 7000B Agilent GC triple-quadrupole and a 5975C Agilent tripleaxis MS detector (Agilent Technologies, CA, USA). A MPS2XL GC-MS autosampler (Gerstal Technologies, Mülheim, Germany) was set to select samples for analysis in a randomised order. MeOX and TMS derivatised samples were injected onto the GC column using a hot needle technique. The injection was operated in splitless (1 µl sample) and split (0.20 µl sample) modes to avoid overloaded chromatogram peaks. The instrumentation conditions and data handling procedures (including mass spectra and peak veri cation processes) were as previously described [19] . Overloaded peaks (lactate, glucose, mannose, sucrose, fructose, urea and cholesterol) were analysed separately from the split mode. Muscle metabolite concentrations (expressed as arbitrary units (AU)) for each metabolite detected in each sample were normalised to the ISTD ( 13 C 6 -Sorbitol) and to muscle sample wet weight.