Recombinant human erbb2 her2 fc chimera protein

Cell-surface expression of the HER2-CAR molecules on PB-HER2-CAR-T cells or CD19-CAR molecules on PB-CD19-CAR-T cells was determined using the flow cytometry using recombinant human ErbB2/HER2-Fc chimera protein or recombinant human CD19 Fc chimera protein, respectively (R&D Systems, Minneapolis, MN, USA), followed by a goat anti-human IgG Fc fragment-specific antibody conjugated to fluorescein isothiocyanate (FITC; Merck Millipore, Burlington, MA, USA). Cell-surface expression of the GD2-CAR molecule on PB-GD2-CAR-T cells was determined using flow cytometry using a goat anti-human IgG Fc fragment-specific antibody conjugated to FITC (Merck Millipore). Phycoerythrin (PE) or allophycocyanin-conjugated antibodies CD3, CD4, CD8, CD45RA, CCR7, and PD-1 (BioLegend, San Diego, CA, USA) were used for the characterization of the phenotype of CAR-T cells. Detailed information of recombinant protein and antibody used was shown in Table S1. All flow cytometry data was acquired using BD Accuri C6 Plus or BD FACSLyric (BD Biosciences) and analyzed using the FlowJo Software (BD Biosciences).