6β hydroxytestosterone

The following are used in the study: testosterone, paracetamol, phenacetin, and NADPH (Beijing Solarbio Science & Technology Co., Ltd. China); dextran, dextromethorphan, omeprazole, 5-hydroxy omeprazole, tolbutamide, 4-hydroxytolbutamide, and 6β-hydroxytestosterone (Toronto Research Chemicals); BCA protein Kit (Lianke Biotechnology Corporate Limited, No. a81911151); primer (synthesized by Tsingke Biotechnology Co., Ltd.); reverse transcription Kit (Tsingke Biotechnology Co., Ltd., No. 20190328); and CYP1A2, cyp2d1, CYP3A4, CYP3A1, CYP2C19, and CYP2C11 antibodies (Abcam Company).

RTV, LPV, and indinavir sulfate were purchased from Macklin Inc. (Shanghai, China); testosterone was purchased from Sigma-Aldrich (St. Louis, MO, United States); 6β-hydroxytestosterone was purchased from Toronto Research Chemicals Inc. (Toronto, Canada); β-nicotinamide adenine dinucleotide phosphate (NADPH) was purchased from Roche (Roche, Switzerland); propranolol was obtained from the National Institutes for Food and Drug Control (Beijing, China); Solutol HS-15 was purchased from MedChemExpress LLC (Shanghai, China); and XYPI was purchased from Jiangxi Qingfeng Pharmaceutical Co. Ltd. (Jiangxi, China). A pooled sample of human liver microsomes (HLM, 452161) was obtained from BD Gentest Corporation (BD Gentest TM, Woburn, MA, United States). Antibodies specific for CYP3A1 (Mouse monoclonal antibody) was purchased from Santa Cruz Biotechnology (Cruz, CA, United States). Primary antibody for GAPDH (Rabbit Polyclonal antibody) and the second-antibody specific for Rabbit and Mouse were purchased from Proteintech Group, Inc. Methanol and acetonitrile of high-performance liquid chromatography (HPLC) grade were obtained from Fisher Co. Ltd. (Waltham, MA, United States). Milli-Q (Millford, MA, United States) water was used throughout the study.

Chlorzoxazone, dextromethorphan, tolbutamide, testosterone, omeprazole, 6-hydroxyChlorzoxazone, dextrorphan, and 5-hydroxyomeprazole were purchased from Sigma-Aldrich Company (St. Louis, USA). 6β-Hydroxytestosterone and 4-hydroxytolbutamide were purchased from Toronto Research Chemicals Inc. (Toronto, Canada). β-Nicotinamide adenine dinucleotide phosphate (NADPH) was purchased from Roche (Roche, Switzerland). Vinpocetine, propranolol, and gliclazide were obtained from the National Institutes for Food and Drug Control (Beijing, China). Acetonitrile of high-performance liquid chromatography grade was obtained from Fisher Co. Ltd. (Waltham, MA, USA). Milli-Q (Milford, MA, USA) water was used throughout the experiments.