2 × 106 cells were washed in FACS buffer (PBS, 10% FBS, and 0.05% sodium azide), and then resuspended in FACS buffer to a concentration of 1 × 106 cells/mL to probe GFP/YFP expression. For surface staining, primary CD4+ T cells were washed in FACs buffer, and then stained with anti-CD44 or anti-CD69 conjugated to Alexa-fluor 647 (Biolegend), CD45RO conjugated to PE-Cy5 (BD Pharmingen). For CD4 staining, cells were first stained with primary anti-CD4 (clone RPA-T4, Biolegend) and then with secondary Alexa 488 (Biolegend). Cells were left on ice for 30 min during staining while gently vortexing every 10 minutes. Cells were washed, and the mean fluorescence intensity (MFI) of each sample was obtained using Accuri C6 flow cytometer.
Anti cd44 or anti cd69 conjugated to alexa fluor 647
Manufactured by BioLegend
Anti-CD44 or anti-CD69 conjugated to Alexa-fluor 647 is a fluorescently-labeled antibody used for flow cytometry analysis. The Alexa-fluor 647 dye enables detection in the far-red region of the spectrum.
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Lab products found in correlation
2 protocols using anti cd44 or anti cd69 conjugated to alexa fluor 647
1
Flow Cytometry Analysis of T Cell Activation
2
Flow Cytometry Analysis of T Cell Activation
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2 × 106 cells were washed in FACS buffer (PBS, 10% FBS, and 0.05% sodium azide), and then resuspended in FACS buffer to a concentration of 1 × 106 cells/mL to probe GFP/YFP expression. For surface staining, primary CD4+ T cells were washed in FACs buffer, and then stained with anti-CD44 or anti-CD69 conjugated to Alexa-fluor 647 (Biolegend), CD45RO conjugated to PE-Cy5 (BD Pharmingen). For CD4 staining, cells were first stained with primary anti-CD4 (clone RPA-T4, Biolegend) and then with secondary Alexa 488 (Biolegend). Cells were left on ice for 30 min during staining while gently vortexing every 10 minutes. Cells were washed, and the mean fluorescence intensity (MFI) of each sample was obtained using Accuri C6 flow cytometer.
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