The NMR spectra were run at 300 and 500 (1H), 75 and 125 (13C) MHz, on Varian Mercury 300 and JEOL GX-500 NMR spectrometers, respectively. The chemical shifts (δ) are reported in ppm downfield to TMS in the appropriate deuterated solvent. For ESI-MS analyses, LCQ (Finnigan MAT 95, Bremen, Germany) and LTQ-FT-MS spectrometers were used, while HR-ESI-MS (Thermo Electron, Finnigan, Germany) was used for HR-ESI-MS analyses. UV spectrophotometer (JASCO V-630) was used for analysis of pure samples in MeOH and in different UV shift reagents.
Material for column chromatography (CC), including microcrystalline cellulose, polyamide S 6 and Sephadex LH-20, as well as Whatman No. 1 sheets used for paper chromatography were purchased from sources described in our previous literature.[11 (link)] Isolated compounds were detected using Naturstoff[11 (link)] and/or FeCl3 (1% in ethanol) spray reagents. Solvent systems S1 (n-BuOH/HOAc/H2O; 4:1:5 v/v/v top layer), S2 (HOAc/H2O; 15:85 v/v) and S3 (n-BuOH/iso-propanol/H2O; 4:1:5, v/v/v top layer) were used. Indomethacin was obtained from Epico, Egypt; paracetamol and silymarin from Sedico, 6th October, Egypt; carrageenan from Sigma, USA; alanine aminotransferase (ALT), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) kits were purchased from Marcy’ Étoile (France).
Material for column chromatography (CC), including microcrystalline cellulose, polyamide S 6 and Sephadex LH-20, as well as Whatman No. 1 sheets used for paper chromatography were purchased from sources described in our previous literature.[11 (link)] Isolated compounds were detected using Naturstoff[11 (link)] and/or FeCl3 (1% in ethanol) spray reagents. Solvent systems S1 (n-BuOH/HOAc/H2O; 4:1:5 v/v/v top layer), S2 (HOAc/H2O; 15:85 v/v) and S3 (n-BuOH/iso-propanol/H2O; 4:1:5, v/v/v top layer) were used. Indomethacin was obtained from Epico, Egypt; paracetamol and silymarin from Sedico, 6th October, Egypt; carrageenan from Sigma, USA; alanine aminotransferase (ALT), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) kits were purchased from Marcy’ Étoile (France).