Hbx sirna

HBx plasmid -pCXN2-HBx of genotype D was gifted by Dr. Tatsuo Kanda, Japan. 1.3 fold full length HBV genome (genotype D) cloned into pUC 19 vector was a generous gift of Dr. Mashashi Mizokami, Japan. RNA was extracted 48 hours post transfection. HBx-siRNA
[24 (link)] was used to produce small interfering RNAs (siRNAs) targeting HBx mRNA (genotype D) (Ambion, TX, USA). siRNA duplexes with non-specific sequences were taken as negative control (NC) for siRNA experiments. siRNA transfection were carried out using Lipofecatmine 2000 (Invitrogen, CA, USA) reagent and medium was replaced 6 hour after transfection. RNA was extracted at 24, 48 and 72 hours post siRNA treatment.

HBx plasmid -pCXN2-HBx was gifted by Dr. Tatsuo Kanda, Japan. 1.3 fold full length HBV genome cloned into pUC 19 vector was a gift of Dr. Mashashi Mizokami, Japan. HBx-siRNA [27 (link)] was used to produce small interfering RNAs (siRNAs) targeting HBx mRNA (Ambion, Texas, USA). siRNA duplexes with non-specific sequences were taken as negative control (NC) for siRNA experiments. siRNA transfection was carried out using Lipofecatmine 2000 (Invitrogen, Carlsbad, CA, USA) reagent and medium was replaced 6 h after transfection. RNA were extracted following different time interval.