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2 protocols using phenol reagent 2 n

1

Phytochemical Analysis Protocol

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Folin–Ciocalteu's phenol reagent 2 N, 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,4,6–Tris (2-pyridyl)-s-triazine (TPTZ), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), aluminium chloride hexahydrate (AlCl3), N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA), quercetin, gallic acid, sodium nitrite, and methanol were acquired from Sigma–Aldrich (St. Louis, MO, USA). Saturated alkanes standard (C7-C40) was purchased from Supelco (Bellefonte, PA, USA). Sodium hydroxide, hydrochloric acid, and ethanol were obtained from J.T. Baker (Phillipsburg, NJ, USA). Sodium carbonate (Na2CO3) was purchased from Fisher Scientific (Lisbon, Portugal), acetic acid was from Panreac (Barcelona, Spain), and ferric chloride (FeCl3) was from Mallinckrodt (New York, NY, USA). Water was purified in a Milli-Q water purification system Millipore (Bedford, MA, USA).
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2

Phytochemical Analysis and Antioxidant Evaluation

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Chemicals and reagents used in this study and their suppliers: Fructose, Maltose, Aniline, Vanillin, Folin and Ciocalteu's Phenol Reagent 2N (Sigma-Aldrich, St. Louis, MO, USA), 4,5,7-Trihydroxyflavanone (Alfa Aesar, England, UK), 2,2-Diphenyl-1-picrylhydrazyl (DPPH*) (Fluka AG, Buchs SG, Switzerland), Anhydrous sodium sulfate and Silica gel 60 F254 HPTLC glass plates (20 cm × 10 cm) (Merck KGaA, Darmstadt, Germany), Boric acid (Pharma Scope, Welshpool, WA, Australia), Glucose, Sucrose, Sodium carbonate anhydrous (Chem-Supply Pty Ltd., St. Gillman, SA, Australia), Gallic acid, Diphenylamine, Phosphoric acid (Ajax Finechem Pvt Ltd., Sydney, Australia).
Solvents used in this study and their suppliers: Methanol (Scharlau, Barcelona, Spain), Dichloromethane (Merck KGaA, Darmstadt, Germany), Ethanol, Ethyl acetate and Formic acid (Ajax Finechem Pvt Ltd., Sydney, Australia), 1-Butanol (Chem-Supply Pty Ltd., St. Gillman, SA, Australia), 2-Propanol and Toluene (Asia Pacific Specialty Chemicals Ltd, Sydney, Australia).
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