Anti b220 pacific blue

Manufactured by Thermo Fisher Scientific

Sourced in Germany

The Anti-B220-Pacific Blue is a fluorochrome-conjugated antibody that binds to the B220 antigen, also known as CD45R, which is expressed on the surface of B cells. This product is intended for use in flow cytometry applications to identify and analyze B cell populations within a sample.

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Lab products found in correlation

Anti foxp3 fitc, by Thermo Fisher Scientific (1 mentions) Anti cd11b alexafluor 700, by Thermo Fisher Scientific (1 mentions) Anti cd8 pe cy7, by Thermo Fisher Scientific (1 mentions) Anti cd4 apc cy7, by Thermo Fisher Scientific (1 mentions) Foxp3 fixation permeabilization working solution, by Thermo Fisher Scientific (1 mentions) Lsr 2 flow cytometer, by BD (1 mentions) Anti cd44 pe, by Thermo Fisher Scientific (1 mentions) Anti cd25 percp cy5, by Thermo Fisher Scientific (1 mentions) Anti cd62l apc mel 14, by BD (1 mentions) Anti cd4 alexa fluor 700 rm4 5, by BD (1 mentions) Facsaria 3, by BD (1 mentions) Facsdiva, by BD (1 mentions) Fc block, by BD (1 mentions) Lsrii flow cytometer, by Tree Star (1 mentions) Anti igm fitc, by Thermo Fisher Scientific (1 mentions) Foxp3 staining buffer set, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-B220 (46 citations)

3 protocols using anti b220 pacific blue

Analysis of Immune Cell Subsets

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For the analysis of cell surface markers, single-cell suspensions from the spleens were prepared 1 week after the last dose of DMBA. Cells were permeabilized by 0.5% (w/v) saponin in PBS (containing 0.25% (w/v) bovine serum albumin and 0.02% (w/v) sodium azide). Splenocytes (macrophages, B cells, CD4⁺ T cells and CD8⁺ T cells) were analyzed by staining with an antibody solution containing anti-CD11b-Alexa Fluor 700, anti-B220-Pacific Blue, anti-CD4-Dye647 and anti-CD8-PE/Cy7 (all obtained from eBioscience, Frankfurt, Germany). Tregs were analyzed by staining with anti-CD4-APC/Cy7 and anti-CD25-Dye647 and subsequent incubation with FoxP3 Fixation/Permeabilization working solution (all obtained from eBioscience). Then cells were stained with anti-FoxP3-FITC (eBioscience). All samples were measured using the flow cytometer BD LSR II (BD Biosciences, San Jose, CA, USA).

Salah F.S., Ebbinghaus M., Muley V.Y., Zhou Z., Al-Saadi K.R., Pacyna-Gengelbach M., O'Sullivan G.A., Betz H., König R., Wang Z.Q., Bräuer R, & Petersen I. (2016). Tumor suppression in mice lacking GABARAP, an Atg8/LC3 family member implicated in autophagy, is associated with alterations in cytokine secretion and cell death. Cell Death & Disease, 7(4), e2205-.

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Multicolor Flow Cytometry Antibody Panel

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The following monoclonal antibodies were used for murine FACS stainings: From BioLegend (San Diego, CA): anti-CD4 Biotin (GK1.5, 1:400), anti-CD8a Pacific Blue (53-6.7; 1:300), anti-CD11b Pacific Blue (M1/70, 1:300), anti-CD11c Brilliant Violett 421 (N418, 1:400), anti-B220 Pacific Blue (RA3-6B2, 1:300), anti-F4/80 Pacific Blue (BM8, 1:400), anti-CD25 PerCP-Cy5.5 (PC61, 1:200), anti-CD44 PE (IM7, 1:800, 1:3000 for analysis with ≤1,000 cells per well), anti-Ki67 APC (16A8, 1:400); anti-Ki67 Brilliant Violett 605 (16A8, 1:400), anti-CD90.1 PerCP-Cy5.5 (OX-7, 1:500), anti-CD90.2 APC-Cy7 (30-H12, 1:500); from eBioscience (San Diego, CA): anti-CD4 Alexa Fluor 700 (RM4-5; 1:200; 1:600 for analysis with ≤1,000 cells), anti-CD62L APC (MEL-14, 1:400), anti-Foxp3 FITC (FJK-16s, 1:200), polyclonal donkey anti-rabbit IgG PE (1:2000); from BD Biosciences: anti-CD14 V450 (rmC5-3, 1:400). Unspecific binding of antibodies was prevented by incubation of cell suspensions with Fc-Block (BD Pharmingen, 2.4G2, 1:100) for 10 min on ice, followed by flow cytometric staining for 30 min on ice in the dark. Cells were passed through a 40 μm cell strainer (NeoLab) to remove large debris. Enumeration of cells and acquisition were performed by using FACSAriaIII and FACSDiva software (BD version 6.1.3). Single-cell data analyses were performed by the use of the FlowJo software 7.6.1 (Tree Star Inc., OR).

Kälin S., Becker M., Ott V.B., Serr I., Hosp F., Mollah M.M., Keipert S., Lamp D., Rohner-Jeanrenaud F., Flynn V.K., Scherm M.G., Nascimento L.F., Gerlach K., Popp V., Dietzen S., Bopp T., Krishnamurthy P., Kaplan M.H., Serrano M., Woods S.C., Tripal P., Palmisano R., Jastroch M., Blüher M., Wolfrum C., Weigmann B., Ziegler A.G., Mann M., Tschöp M.H, & Daniel C. (2017). A Stat6/Pten axis links cold exposure with T cell tolerance in adipose tissue. Cell metabolism, 26(3), 475-492.e7.

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B Cell Characterization by Flow Cytometry

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Cells were stained for surface antigens using anti-B220-PacificBlue, anti-IgM-FITC, anti-CD43-APC (all from eBioscience, San Diego, CA). Then, a fixation/permeabilization procedure was performed using the Foxp3 Staining Buffer Set (eBioscience) according to the manufacturer’s protocol, followed by staining with anti-TdT-PE (19−3; eBioscience). Cells were analysed with an LSR II flow cytometer with FlowJo (TreeStar Inc., Ashland, OR, USA) software.

Nguyen T.V., Pawlikowska P., Firlej V., Rosselli F, & Aoufouchi S. (2016). V(D)J recombination process and the Pre-B to immature B-cells transition are altered in Fanca−/− mice. Scientific Reports, 6, 36906.

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