Phospholipids 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC; 850375C), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE; 850725C), DOPA (840875C), and 18:1-12:0 1-oleoyl-2-(12-[(7-nitro-2-1,3-benzoxadiazol-4-yl) amino] dodecanoyl)-sn-glycero-3-phosphate, (NBD-PA; 810176C) were obtained from Avanti Polar Lipids. Rhodamine DHPE (L-1392) was purchased from ThermoFisher Scientific. Lipids in chloroform-containing stock solutions were mixed at the desired molar ratio, and the solvent was evaporated. The lipid film was hydrated in a suitable buffer, and the suspension was incubated at 37 °C for 30 min and then frozen in liquid nitrogen. Five freeze-thaw cycles were carried out and the thawed solution was extruded 21 times through a 0.1-µm filter, using a mini-extruder (Avanti Polar Lipids).
Rhodamine dhpe l 1392
Manufactured by Thermo Fisher Scientific
Rhodamine DHPE (L-1392) is a fluorescent lipid probe used in cell and membrane research. It consists of a rhodamine fluorophore coupled to a dipalmitoyl phosphoethanolamine (DHPE) lipid. The product can be used to label and track lipid bilayers, membranes, and lipid-containing structures.
Automatically generated - may contain errors
Lab products found in correlation
Mini extruder, by Avanti Polar Lipids (1 mentions)
Nbd pa, by Avanti Polar Lipids (1 mentions)
Mitotracker green fm, by Thermo Fisher Scientific (1 mentions)
Brain pi 4 5 p2, by Avanti Polar Lipids (1 mentions)
1 palmitoyl 2 oleoyl sn glycero 3 phosphatidylserine, by Avanti Polar Lipids (1 mentions)
2 protocols using rhodamine dhpe l 1392
1
Phospholipid Vesicle Preparation Protocol
2
Lipid Membrane Composition and Probes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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1-Palmitoyl-2-oleoyl-sn-glycero-3-PC (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-PE (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylserine (POPS), cardiolipin, and brain PI(4,5)P2 were purchased from Avanti Polar Lipids. Rhodamine DHPE (L-1392) and DPH were from Thermo Fisher Scientific. MitoTracker green FM and Red CMXRos were from Thermo Fisher Scientific.
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