Chitosan

Chitosan produced from Aspergillus niger (Deacetylation degree (DD) = 92%, determined by FT-IR method; Mw = 890,000 g/mol; and 100–300 cps), fluorescein, L-aspartic, and L-glutamic acid were purchased from PolAura, Dywity, Poland. Propylene glycol, isopropanol, methanol (HPLC grade), (CH₃COO)₂Zn∙2H₂O, CaCl₂·2H₂O, MgSO₄, KCl, KOH, ethanol (96%, 99.9%), KHPO₄, Na₂HPO₄, NaHCO₃, NaCl, HCl, NaOH, mouse L929 (commercial cell line from mouse C3H/An connective tissue for research purpose only, The European Collection of Authenticated Cell Cultures (ECACC)), Dulbecco’s Modified Eagle Medium (DMEM) with glucose content cell culture medium, antibiotics (streptomycin/penicillin), fetal bovine serum (FBS), phosphate buffer solution (PBS), trypsin with EDTA, and Triton-X-100 were purchased from Sigma Aldrich, Poznań, Poland. For the study, pure cannabidiol of HPLC confirmed purity was used, purchased from Dobre Konopie, Mysłowice, Poland.

Chitosan of average molecular mass = 300,000 g/mol was purchased from PolAura (Dywity, Poland). Poly (lactic acid), acetone (analytical grade), acetonitrile, trifluoroacetic acid, NaCl, (CH₃COO)₂Zn 2H₂O, NaHCO₃, KCl, K₂HPO₄ 3H₂O, MgCl₂ 6H₂O, KOH, HCl, CaCl₂, chloroauric acid, Na₂SO₄, (CH₂OH)₃CNH₂, methanol, ethanol, XTT assay, L929 mouse fibroblasts cell line, human dermal fibroblasts (HDF) primary cells, fibroblasts complete growth medium (FGM), Dulbecco’s Modified Eagle Medium (DMEM) with high glucose content, phosphate buffer solution (PBS), streptomycin/penicillin, trypsin, and FGF-2/basic FGF Protein were purchased from SigmaAldrich (Poznań, Poland). All other reagents were of analytical grade and purchased from SigmaAldrich.