The Human Chemokine Array C1 (RayBiotech, Inc.) was used to measure chemokine levels within the exosomes. Briefly, 100 µg exosomes were lysed in lysis buffer and analyzed according to the manufacturer's instructions. The densitometric analysis of the arrays was performed using the Multi Gauge (version 3.11; Fuji Film).
Human chemokine array c1
Manufactured by RayBiotech
Sourced in United States
The Human Chemokine Array C1 is a multiplex assay that allows for the simultaneous detection and semi-quantitative measurement of 80 different human chemokines in a single sample. The array utilizes a capture-based approach to provide a comprehensive profile of chemokine expression.
Automatically generated - may contain errors
4 protocols using human chemokine array c1
1
Exosomal Chemokine Profiling
2
Human Chemokine Array Evaluation
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Human Chemokine Array C1 from RayBiotech (Atlanta, GA, USA) contains 38 repeated antibodies spotted on a single membrane. Experiments were implemented according to the manufacturer’s protocol, and the results were analyzed by both Lane 1D software from Sage Creation (Beijing, China) and software from RayBiotech.
3
Breast Cancer Cells Crosstalk Profiling
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Co-culture was performed using transwell chamber system (0.4-μm pores, BD Falcon, USA) in 24-well dish, in which 1 × 105 human or mouse breast cancer cells were seeded in the bottom well while 1 × 105 THP-1, U937, or mouse monocytes were seeded in the upper chamber. After 48 h incubation, the media was harvested and centrifuged at 1200 rpm to remove the cell pellet. Human Chemokine Array (C1; RayBiotech, USA) was then applied to detect differential chemokine expressions. The chemokine array experiments were carried out in duplicate.
4
Antibody Arrays for Angiogenesis and Chemokines
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Human Angiogenesis Antibody Array C1000 and Human Chemokine Array C1 were purchased from RayBiotech (Norcross, GA, USA). Human Angiogenesis Antibody Array C1000 kit was consisted of 43 different antibodies spotted in duplicate onto two membranes. Human Chemokine Array C1 Kit was consisted of 38 different antibodies spotted in duplicate onto one membrane. Experiments were done as recommended by the manufacturer. Angiogenesis and Chemokine array were analyzed by imagine QTL software from GE and software from RayBiotech.
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