Pancreas tissue samples were obtained from the db/db mice, fixed in 4% formaldehyde solution, and then embedded in paraffin. Paraffin sections (5-µm-thick) were used for immunohistochemical and morphometric analyses. For pancreatic polypeptide (PPY) and somatostatin (SST) positivity determination, the mean optical density (MOD) of islet PPY and SST were measured and calculated using Image-Pro Plus 6.0 software. insulin and glucagon were studied by immunofluorescence staining, which was performed as previously described. The following primary antibodies were used: somatostatin (rabbit polyclonal, Abcam), pancreatic polypeptide (goat polyclonal, Abcam), insulin (guinea pig polyclonal, Abcam), and glucagon (goat polyclonal, Abcam).
Pancreatic polypeptide
Manufactured by Abcam
Sourced in Japan
Pancreatic polypeptide is a lab equipment product. It is a hormone produced by the pancreas that plays a role in the regulation of appetite and energy metabolism.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pancreatic polypeptide
1
Pancreatic Morphometry and Immunohistochemistry
2
Immunohistochemical Analysis of Tumor Markers
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Tissue sections were prepared and subjected to hematoxylin and eosin (H&E) staining for observation under a light microscope. Antibodies against the following were then used via immunohistochemically evaluating the three tumor cell types: Bcl-2 (1:50 dilution; Dako Japan, Tokyo, Japan, Clone name: 124), CD56 (1:100 dilution; Novocastra Newcastle upon Tyne, UK, Clone name: 1B6), chromogranin A (1:800 dilution; Dako Japan, Clone name: DAK-A3), estrogen receptor (Ready to Use; Roche Diagnostics Co., Tokyo, Japan, Clone name: SP1), Ki-67 (1:200 dilution; Dako Japan, Clone name: MIB-1), pan-cytokeratin (1:400 dilution; Dako Japan, Clone name: AE1/AE3), pancreatic polypeptide (1:100 dilution; Abcam, Cambridge, UK, incubated with Histofine Simple Stain MAX-PO (G) (Nichirei Bioscience, Tokyo, Japan), polyclonal), progesterone receptor (Ready to Use; Roche Diagnostics Co., Clone name: 1E2), somatostatin (Ready to use; Dako Japan, polyclonal), synaptophysin (1:40 dilution; Dako Japan, Clone name: M0776), and S-100 protein (1:2400 dilution; Dako Japan, polyclonal).
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