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Rabbit anti gr

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Rabbit anti-GR is a primary antibody directed against the glucocorticoid receptor (GR). It is used in various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to detect and study the expression and localization of the GR protein.

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Lab products found in correlation

Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Cy3 or fitc conjugated secondary antibody, by Jackson ImmunoResearch (2 mentions) Rat anti mbp, by Abcam (2 mentions) Anti rabbit cy5, by Jackson ImmunoResearch (2 mentions) Anti rat fitc, by Jackson ImmunoResearch (2 mentions) Anti mouse cy3, by Jackson ImmunoResearch (2 mentions) Mouse anti β 3 tubulin tuj1, by Fortrea (2 mentions) Mouse anti nestin, by BD (2 mentions) Bz 9000, by Keyence (1 mentions) Mouse anti galectin 1, by Santa Cruz Biotechnology (1 mentions) Mouse anti gapdh antibody, by Merck Group (1 mentions) Ecl kit, by Cytiva (1 mentions) Rabbit anti actin a2066, by Merck Group (1 mentions) Fitc or cy3 labeled secondary antibodies, by Jackson ImmunoResearch (1 mentions) Ab6640, by Abcam (1 mentions) Te 1000, by Nikon (1 mentions) Vp k451, by Vector Laboratories (1 mentions) Rabbit anti α sma, by Merck Group (1 mentions) Most00, by R&D Systems (1 mentions)

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Anti-GR (6 citations)

6 protocols using rabbit anti gr

1

Immunofluorescence Staining of Protein Markers

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Immunofluorescence analyses were performed as described previously.4, 5, 15 Serial sections were incubated with the following primary antibodies: mouse anti‐galectin‐1 (1:50, Santa Cruz Biotechnology), mouse and rabbit anti‐glial fibrillary acidic protein (GFAP) (1:200, Leica), rabbit anti‐phosphorylated ATF2 (1:100), rabbit anti‐phosphorylated c‐Fos (1:100), and rabbit anti‐phosphorylated c‐Jun (1:100, Cell Signaling Technology), rabbit anti‐DUSP1 (1:100, Millipore) and rabbit anti‐GR (1:100, Thermo Fisher Scientific) antibodies. Secondary antibodies for fluorescent detection were AlexaFluor 488 and 546 (1:500, Thermo Fisher Scientific). Nuclei were counterstained with DAPI, and sections were visualized under a Keyence BZ‐9000 (Keyence).
Hirose I., Kanda A., Noda K, & Ishida S. (2019). Glucocorticoid receptor inhibits Müller glial galectin‐1 expression via DUSP1‐dependent and ‐independent deactivation of AP‐1 signalling. Journal of Cellular and Molecular Medicine, 23(10), 6785-6796.
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2

Immunostaining of Neural Stem Cells

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NSCs were fixed in 4% paraformaldehyde (PFA) and immunostained with rabbit anti-GR (1:500, Thermo Scientific, Rockford IL), mouse anti-Nestin (1:500, BD Biosciences, San Jose CA), and rat anti-BrdU (1:500) overnight at 4°C, and then treated with Cy3 anti-mouse, FITC anti-rat, and Cy5 anti-rabbit (1:500, Jackson ImmunoResearch, West Grove PA) for 2h.
For differentiation studies, cells were immunostained (overnight, 4°C) with mouse anti-β-III tubulin (Tuj1; 1:1000, Covance, Princeton NJ) or rat anti-MBP (1:100, Abcam, Cambridge MA) followed by 2h incubation with Cy3 or FITC-conjugated secondary antibodies (1:500, Jackson). The nuclear stain DAPI was added prior to mounting coverslips in 1,4-diazabicyclo[2.2.2]octane (DABCO). Images were obtained with an inverted fluorescence microscope (20× objective; Zeiss, Oberkochen, Germany), and blind cell counts were taken using Metamorph software. 20 random visual fields were analyzed per coverslip. Data are the percentage of positive cells in relation to the total number of DAPI-stained nuclei present in the culture. All experiments were independently replicated at least 3 times.
Chetty S., Friedman A.R., Taravosh-Lahn K., Kirby E.D., Mirescu C., Guo F., Krupik D., Nicholas A., Geraghty A., Krishnamurthy A., Tsai M.K., Covarrubias D., Wong A., Francis D., Sapolsky R.M., Palmer T.D., Pleasure D, & Kaufer D. (2014). Stress and glucocorticoids promote oligodendrogenesis in the adult hippocampus. Molecular psychiatry, 19(12), 1275-1283.
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3

Immunostaining of Neural Stem Cells

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NSCs were fixed in 4% paraformaldehyde (PFA) and immunostained with rabbit anti-GR (1:500, Thermo Scientific, Rockford IL), mouse anti-Nestin (1:500, BD Biosciences, San Jose CA), and rat anti-BrdU (1:500) overnight at 4°C, and then treated with Cy3 anti-mouse, FITC anti-rat, and Cy5 anti-rabbit (1:500, Jackson ImmunoResearch, West Grove PA) for 2h.
For differentiation studies, cells were immunostained (overnight, 4°C) with mouse anti-β-III tubulin (Tuj1; 1:1000, Covance, Princeton NJ) or rat anti-MBP (1:100, Abcam, Cambridge MA) followed by 2h incubation with Cy3 or FITC-conjugated secondary antibodies (1:500, Jackson). The nuclear stain DAPI was added prior to mounting coverslips in 1,4-diazabicyclo[2.2.2]octane (DABCO). Images were obtained with an inverted fluorescence microscope (20× objective; Zeiss, Oberkochen, Germany), and blind cell counts were taken using Metamorph software. 20 random visual fields were analyzed per coverslip. Data are the percentage of positive cells in relation to the total number of DAPI-stained nuclei present in the culture. All experiments were independently replicated at least 3 times.
Chetty S., Friedman A.R., Taravosh-Lahn K., Kirby E.D., Mirescu C., Guo F., Krupik D., Nicholas A., Geraghty A., Krishnamurthy A., Tsai M.K., Covarrubias D., Wong A., Francis D., Sapolsky R.M., Palmer T.D., Pleasure D, & Kaufer D. (2014). Stress and glucocorticoids promote oligodendrogenesis in the adult hippocampus. Molecular psychiatry, 19(12), 1275-1283.
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4

Western Blot Analysis of Liver and Kidney Proteins

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Liver or kidney extracted proteins (25 μg homogenate) were resolved on 10% polyacrylamide gels by SDS-PAGE electrophoresis, transferred to a nitrocellulose membrane, and then incubated with a GR-specific antibody ((rabbit anti-GR (N-terminal, M-20; Santa Cruz, CA, USA), rabbit anti-GR (C-terminal amino-acids 755–771, PA1-516, Thermo Fischer Scientific)). sEH expression was analysed in 30 µg of protein lysate (rabbit anti-sEH, Cayman Chemicals, 10010146). Immunoreactive bands were visualized by chemiluminescence (ECL kit; Amersham Biosciences, 152 Little Chalfont, UK). An anti-actin monoclonal (rabbit anti-actin (A2066, Merck)) or a mouse anti-GAPDH antibody (Merck) were used to verify equal protein loading between samples.
Vanderriele P.E., Wang Q., Mérillat A.M., Ino F., Aeschlimann G., Ehret X., Ancin Del Olmo D., Ponce de León V., Scholl U.I., Winter D.V., Odermatt A., Hummler E, & Verouti S.N. (2021). Salt-Sensitive Hypertension in GR+/− Rats Is Accompanied with Dysregulation in Adrenal Soluble Epoxide Hydrolase and Polyunsaturated Fatty Acid Pathways. International Journal of Molecular Sciences, 22(24), 13218.
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5

Immunofluorescence Analysis of Kidney Markers

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Immunofluorescence staining of the kidney was performed on fixed-frozen sections. Briefly, the tissue sections were activated and labeled with antibodies, including rabbit anti-Ki-67 (Vector, VP-K451, 1 in 200), rabbit anti-KIM-1 (R925 , 1 in 200), rabbit anti-α-SMA (Sigma, 1 in 400), rat anti-F4/80 (Abcam, ab6640, 1 in 1000), rabbit anti-Hnf1b (Thermo Fisher Scientific, 720259), rabbit anti-GR (Thermo Fisher Scientific, PA1-511A), rabbit anti-Slc34a1 (Novusbio, NBP2-13328), rabbit anti-Spp1 (Abcam, ab8448) and rat anti-Kl (BioLogo, KM2076). The slides were then exposed to FITC or Cy3-labeled secondary antibodies (Jackson ImmunoResearch). The staining was examined with fluorescence microscopes (Nikon TE 1000 and Nikon C1 confocal). At least 7 high-power fields/section for each sample were examined in each evaluation and quantification (positive area or cell count) was performed with an in-house Macro in Image J. Spp1 plasma concentration was measured by ELISA (R&D Systems, MOST00).
Wilflingseder J., Willi M., Lee H.K., Olauson H., Jankowski J., Ichimura T., Erben R., Valerius M.T., Hennighausen L, & Bonventre J.V. (2020). Enhancer and super-enhancer dynamics in repair after ischemic acute kidney injury. Nature Communications, 11, 3383.
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6

Fluorescent Immunohistochemistry of GR and MR in Mouse Brain

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For GR and MR analysis, mice were perfused (transcardially with 4% PFA in PBS, pH 7.4). Brains were kept in fixation solution overnight at 4 °C, then transferred to 30% sucrose solution for 24 h, sectioned (50 μm thickness) on a cryostat and stained while free-floating. Antibodies were as follows: 1:100 NR3C2 monoclonal antibody (ThermoFisher Scientific, MA1–620), 1:200 rabbit anti-GR (ThermoFisher Scientific, PA1–511A). Secondary antibodies were Alexa Fluor 488 (ThermoFisher Scientific, A-21202), 647 (ThermoFisher Scientific, A-31573). dorsal DG, dorsal CA3, and dorsal CA1 were analyzed at −1.43 to −1.79 mm from bregma. PVH was analyzed at −0.59 to −0.95 mm from bregma. 3–5 sections per mouse were acquired and analyzed and the data per mouse was the average of the sections. All the samples were acquired using the same settings (laser power, 20% for MR, 10% for GR, optical slice, 1 μm) on an Leica SP8 on HC PL APO 40×/1.30 oil-immersion. Images were quantified using spot detection software (Imaris 9.2, Bitplane AG, expected radius 1 μm).
Hong J.Y., Lim J., Carvalho F., Cho J.Y., Vaidyanathan B., Yu S., Annicelli C., Eddie W.K, & Medzhitov R. (2020). Long-term programming of CD8 T cell immunity by perinatal exposure to glucocorticoids. Cell, 180(5), 847-861.e15.
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