At the end of the MRI experiments, the animals were sacrificed by cervical dislocation, in agreement with ethical rules. Cancer tissues, kidneys, liver, spleen, and muscles were explanted, weighed, and processed for ICP-MS analysis [53 (link)]. The tissues were added to 1 mL of concentrated HNO3 (70%). Upon dissolution of the tissues, samples were further digested through microwave heating (MicroSYNTH, Microwave labstation, Microsynth, Balgach, Switzerland, equipped with an optical fiber temperature control and HPR-1000/6M high-pressure reactor, Milestone, Bergamo, Italy). After digestion, ultrapure water was added to each sample up to a 2 mL total volume, then samples were filtered with 0.45 μm filter and analyzed using ICP-MS for quantification of Gd3+, using a Thermo Scientific ELEMENT 2 ICP-MS-Finnigan, Rodano, Italy. A calibration curve obtained by using four gadolinium absorption standard solutions (Sigma-Aldrich, Burlington, MA, USA) in the range 0.005–0.1 μg/mL was used for the quantification. The total mass of Gd3+ retained in each specimen was calculated with respect to the weight of the tumor tissue (as μg of Gd3+/g of tissue).
Gadolinium absorption standard solutions
Manufactured by Merck Group
Sourced in United States
Gadolinium absorption standard solutions are aqueous solutions containing known concentrations of gadolinium ions. They are used as standards for the calibration and validation of analytical techniques that measure gadolinium absorption, such as spectroscopic methods.
Automatically generated - may contain errors
2 protocols using gadolinium absorption standard solutions
1
Quantification of Gadolinium in Tissues
2
Quantification of Gadolinium Retention in Tumors
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After the MRI experiments were completed, the animals were sacrificed and the tumors were explanted, weighed and processed for ICP-MS analysis using reported procedures. 11 The explanted tissue was treated by adding 1 mL of concentrated HNO3 (70%) to each sample. After the complete dissolution of the tissues, the samples were further digested using microwave heating (MicroSYNTH, Microwave labstation equipped with an optical fiber temperature control and HPR-1000/6M six position high-pressure reactor, Milestone, Bergamo, Italy). After digestion, the volume of each sample was brought to 2 mL using ultrapure water. The solutions were then filtered with a 0.45 m filter and analyzed by ICP-MS for the quantification of Gd 3+ , using a Thermo Scientific ELEMENT 2 ICP-MS-Finnigan, Rodano (MI). Quantification was obtained using a calibration curve that was measured using four gadolinium absorption standard solutions (Sigma-Aldrich) in the range 0.005-0.1 g/mL. The total mass of the Gd 3+ that was retained in each specimen was calculated with respect to the weight of the tumor tissue (as g of Gd 3+ /g of tissue).
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