Wasp dt walk away specimen processor

Prior to automated systems, 1 μL urine specimens were cultured using the WASP®DT: Walk-Away Specimen Processor (Copan Diagnostics, Murrieta, CA) on Brilliance UTI agar (Oxoid Ltd., Basingstoke, United Kingdom). The cultures, incubated at 35°C for 18–24 h, were considered as positive if growth ≥10⁵ CFUs/ml. We used MALDI-TOF analysis (MALDI Microflex LT, Bruker Daltonics, Bremen, Germany) to identify growing colonies. If there were three or more different types of colonies, the urine was considered as contaminated, although we classified it as negative for research purposes and it was not submitted to the identification procedure.

Prior to flow cytometry, the urine specimens were cultured using a WASP^®DT: Walk-Away Specimen Processor (Copan Diagnostics, Murrieta, CA, USA) on Brilliance UTI agar (Oxoid Ltd., Basingstoke, United Kingdom). Cultures were incubated at 35°C for 18–24 h. Bacterial counts were expressed as the number of colony-forming units per millilitre. Growth of ≥10⁵ CFUs/mL was considered positive. Grown colonies were identified by MALDI-TOF (MALDI Microflex LT, Bruker Daltonics, Bremen, Germany). If there were three or more types of colonies without a dominant species, the urine culture was considered as contaminated but classified as negative and not subjected to the identification procedure.