The LDH toxicity assay kit (Abcam Cat. # ab65393) was used according to the manufacturer's specification. Briefly, released LDH in culture supernatants of Huh7.5 cells after 24 h co-culture with different concentration of Bafilomycin A1 and Lansoprazole was measured as the indicator of lysed cells. The percentage of cytotoxicity was measured by subtracting LDH content in remaining viable cells from total LDH in untreated controls. Staurosporine (20 nM) (Abcam, Cambridge, MA) treatment of Huh7.5 cells for 12 h was used as positive control. The final absorbance was measured at 490 nm. All experiments were performed in triplicate.
Ldh toxicity assay kit
Manufactured by Abcam
The LDH toxicity assay kit is a laboratory tool used to measure the activity of lactate dehydrogenase (LDH) enzyme in biological samples. LDH is released into the extracellular environment upon cell damage or death, making it a useful marker for cytotoxicity or cell viability assays.
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2 protocols using ldh toxicity assay kit
1
LDH Cytotoxicity Assay for Huh7.5 Cells
2
LDH Assay for Versican siRNA Cytotoxicity
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The LDH toxicity assay kit (Abcam Cat. # ab65393) was used according to the manufacturer's specification as recently described. 78 Briefly, released LDH in culture supernatants of LX2 cells was measured after versican siRNA knockdown. Staurosporine (Cell Signaling cat. #9953) treatment (10 nM) for 12 h was used to initiate apoptosis as a positive control for poly(ADP-ribose) polymerase cleavage. The percentage of cytotoxicity was measured by subtracting LDH content in remaining viable cells from total LDH in untreated controls. The final absorbance was measured at 490 nm. All experiments were performed in triplicate with an equal number of cells (5 × 10 6 cells/2 ml cell culture medium).
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