We implement western blot to elucidate the underlying mechanisms by which Pino ameliorated PIHF. The procedures of protein sample extraction and preparation resembled those previously reported in our study (Chen et al. 2020 (link)a). The membranes were probed with antibodies against the nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1; 1:2000, abcam, ab189491), p53 (1:1000, cell signaling technology, 2524S), bcl-2 (1:1000, cell signaling technology, 2870P), bax (1:1000, abcam, ab32503), cleaved caspase-3 (1:1000, cell signaling technology, 9664 T), collagen I (1:1000, Affinity, AF7001), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Servicebio, GB12002), with subsequent visualization by HRP-labeled secondary antibodies (Servicebio, G1213 and G1214) and chemiluminescence detection reagents. The blot images were finally analyzed and quantified by Image J software.
Ab189491
Manufactured by Cell Signaling Technology
Ab189491 is a primary antibody that specifically recognizes the target protein. It is intended for use in various laboratory applications, such as immunoassays and immunohistochemistry, to detect and analyze the target protein.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh, by Wuhan Servicebio Technology (2 mentions)
Ab32503, by Cell Signaling Technology (2 mentions)
G1213, by Wuhan Servicebio Technology (2 mentions)
G1214, by Wuhan Servicebio Technology (2 mentions)
Af7001, by Affinity Biosciences (1 mentions)
Gb12002, by Wuhan Servicebio Technology (1 mentions)
2 protocols using ab189491
1
Western Blot Analysis of Pino's Mechanisms in PIHF
2
Elucidating Pino's Mechanisms in PIHF via Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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We implement western blot to elucidate the underlying mechanisms by which Pino ameliorated PIHF. The procedures of protein sample extraction and preparation resembled those previously reported in our study (23) . The membranes were probed with antibodies against the nuclear factor erythroid 2-related factor 2 (Nrf2, ), heme oxygenase-1 (HO-1; 1:2000, abcam, ab189491), p53 (1:1000, cell signaling technology, 2524S), bcl-2 (1:1000, cell signaling technology, 2870P), bax (1:1000, abcam, ab32503), cleaved caspase-3 (1:1000, cell signaling technology, 9664T), collagen (1:1000, A nity, AF7001), glyceraldehyde-3phosphate dehydrogenase (GAPDH, Servicebio, GB12002), with subsequent visualization by HRP-labeled secondary antibodies (Servicebio, G1213 and G1214) and chemiluminescence detection reagents. The blot images were nally analyzed and quanti ed by Image J software.
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