All UV–Vis absorption and fluorescence spectra were recorded on a UV–Vis Spectrophotometer (Agilent Technologies Cary 300, Santa Clara, CA, USA) and a Spectrofluorometer (PerkinElmer LS55, Waltham, MA, USA), respectively. In both experiments, the stock solutions of chalcones 3a –f were prepared as 0.5 mM in DMSO. The appropriate amount of the stock solution was added to DMSO, MeOH, and 0.01 M PBS buffer (pH 7.4) with 3% Tween 80 (3 mL) to obtain the final concentration of 2 µM in the testing solutions. For fluorescence experiments, the emission spectra were recorded at the maximum absorbance wavelength of each compound. The fluorescent quantum yields (Φf) were calculated relative to fluorescein in 0.1 M NaOH as a standard (Φf = 0.95).
Cary 300
Manufactured by PerkinElmer
Sourced in United States
The Cary 300 is a UV-Vis spectrophotometer designed for laboratory use. It measures the absorption or transmission of light through a sample across a range of ultraviolet and visible wavelengths.
Automatically generated - may contain errors
Lab products found in correlation
Tlc silica gel 60 f254, by Merck Group (2 mentions)
Microtof, by Bruker (2 mentions)
Nanodrop 2000 spectrophotometer, by Thermo Fisher Scientific (2 mentions)
500 mhz nmr, by Bruker (1 mentions)
Bacterial dna extraction kit, by Vivantis Technologies (1 mentions)
500 mhz spectrometer, by Bruker (1 mentions)
4 protocols using cary 300
1
Photophysical Properties of Chalcones
2
Spectroscopic Characterization of Organic Compounds
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The 1H- and 13C-NMR data were collected on a Bruker 500 MHz NMR (Bruker Ltd., Rheinstetten, Germany) in CDCl3, DMSO-d6, and acetone-d6 as solvents at room temperature, with tetramethylsilane (TMS) as an internal reference. High-resolution mass spectra (HRMS) were recorded by an electrospray ionization mass (ESI-MS) spectrometer (MicrOTOF, Bruker, Rheinstetten, Germany). The absorbance and fluorescence spectra were measured on a UV–Vis spectrophotometer (Agilent Technologies Cary 300, CA, USA) and a fluorescence spectrophotometer (PerkinElmer LS55, MA, USA), respectively. All glassware was oven-dried prior to use. All reagents and solvents were purchased from the companies Sigma Aldrich and Merck (MO, USA), TCI (Tokyo, Japan), or Carlo Erba (Barcelona, Spain)and used without further purification. Analytical thin-layer chromatography (TLC) was performed on TLC Silica gel 60 F254 (Merck, MO, USA) and visualized under a UV cabinet lamp.
3
Photophysical Characterization of AZB-IMC2 Probe
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All the UV/vis absorption spectra and fluorescence were recorded on UV-vis Spectrophotometer (Agilent Technologies Cary 300) and Spectrofluorometer (PerkinElmer LS55), respectively. In both experiments, stock solutions (350 μM) of AZB-IMC2 probe was prepared in DMSO. 10 μM solutions of probes in chloroform, DMSO, DI water, and 0.01 M PBS buffer (pH 7.4) with 3% DMSO were prepared as working solutions. For fluorescence experiments, the emission spectra were recorded at excitation wavelength of 670 nm.
4
Analytical Techniques for Organic Compound Characterization
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All chemicals and reagents purchased from commercial sources (Sigma Aldrich, TCI, and Merck) were used without further purification. Analytical thin layer chromatography (TLC) was performed on TLC Silica gel 60 F254 (Merck) and visualized under a UV cabinet. Column chromatography purification was performed using silica gel (Carlo Erba) as a stationary phase for chromatography. UV-vis absorption and fluorescence spectra were recorded using a UV-vis spectrophotometer (Agilent Technologies Cary 300, CA, USA) and a fluorescence spectrophotometer (PerkinElmer LS55, Waltham, MA, USA), respectively. Nuclear magnetic resonance (NMR) spectroscopy (1H and 13C NMR) was recorded on a Bruker 500 MHz spectrometer at room temperature, with TMS as an internal reference. The chemical shifts of the 1H NMR spectra were recorded and reported in ppm using the solvent resonance (DMSO at 2.54 ppm). 13C NMR spectra were also recorded in ppm using the solvent resonance (DMSO at 40.45 ppm). High-resolution mass spectroscopy (HRMS) spectra were recorded on an ESI-MS spectrometer (MicrOTOF, Bruker, Rheinstetten, Germany). The genomic DNA of E. coli 789 was isolated using a bacterial DNA extraction kit (Vivantis) according to the manufacturer's instructions and used as dsDNA, whose concentration was determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific), in the study.
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