Lymphocytes were all stained with the anti-mouse Abs CD4 PE-F594 (BD Biosciences, San Jose, CA), CD4 FITC (BioLegend, San Diego, CA), CD11b AF-700 (BioLegend), and GL3 (γδ TCR). All cytokine staining was performed using the BD Foxp3 intranuclear transcription factor staining kit for Foxp3 PE (eBioscience), T-bet PE (BioLegend), IL-17a Rat PE-Cy7 (BioLegend), IL-17a Alexa Fluor 488 (BD Biosciences), IFN-γ PerCP-Cy5.5 (BD Biosciences), IFN-γ allophycocyanin-Cy7 (BioLegend), IL-10 allophycocyanin (BioLegend), and IL-6 allophycocyanin (BioLegend) following the manufacturer’s instructions. For phenotypic analysis, flow cytometry was performed using a BD Biosciences LSR II flow cytometer, and analysis was performed using FlowJo (Tree Star, Ashland, OR).
Ifn γ allophycocyanin cy7
Manufactured by BioLegend
IFN-γ allophycocyanin-Cy7 is a fluorescent-conjugated antibody that binds to interferon-gamma (IFN-γ). It is used in flow cytometry applications to detect and quantify IFN-γ-producing cells.
Automatically generated - may contain errors
Lab products found in correlation
Cd4 fitc, by BioLegend (2 mentions)
Ifn γ percp cy5, by BD (2 mentions)
Lsrii flow cytometer, by Tree Star (2 mentions)
Cd11b af700, by BioLegend (2 mentions)
T bet pe, by BioLegend (2 mentions)
Cd4 pe f594, by BD (2 mentions)
Il 17a rat pe cy7, by BioLegend (2 mentions)
Il 17a alexa fluor 488, by BD (2 mentions)
Il 10 allophycocyanin, by BioLegend (2 mentions)
Il 6 allophycocyanin, by BioLegend (2 mentions)
2 protocols using ifn γ allophycocyanin cy7
1
Comprehensive Cytokine and Lymphocyte Analysis
2
Comprehensive Cytokine and Lymphocyte Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Lymphocytes were all stained with the anti-mouse Abs CD4 PE-F594 (BD Biosciences, San Jose, CA), CD4 FITC (BioLegend, San Diego, CA), CD11b AF-700 (BioLegend), and GL3 (γδ TCR). All cytokine staining was performed using the BD Foxp3 intranuclear transcription factor staining kit for Foxp3 PE (eBioscience), T-bet PE (BioLegend), IL-17a Rat PE-Cy7 (BioLegend), IL-17a Alexa Fluor 488 (BD Biosciences), IFN-γ PerCP-Cy5.5 (BD Biosciences), IFN-γ allophycocyanin-Cy7 (BioLegend), IL-10 allophycocyanin (BioLegend), and IL-6 allophycocyanin (BioLegend) following the manufacturer’s instructions. For phenotypic analysis, flow cytometry was performed using a BD Biosciences LSR II flow cytometer, and analysis was performed using FlowJo (Tree Star, Ashland, OR).
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