A 16-channel array (35-μm tungsten wires, 150-μm spacing between wires, 150-μm spacing between rows, Innovative Physiology) was epoxied to a fiber optic and lowered into the LC of a lightly (<1% isoflurane) anesthetized, Th-CreLC:ChR2 or Crh-CreCeA-LC:ChR2 animals. Voltages from each electrode were bandpass-filtered with activity between 250 and 8,000 Hz analyzed as spikes. LC cells were selected based on stereotaxic position, baseline activity, and toe pinch response. The signal was amplified and digitally converted (Omniplex and PlexControl, Plexon), spikes were sorted using principal component analysis and/or evaluation of t-distribution with expectation maximization (Offline sorter, Plexon). Sorted units were analyzed with NeuroExplorer 3.0 and timestamps were exported for further analysis in Microsoft Excel and Matlab 7.12.
Plexcontrol
Manufactured by Plexon
PlexControl is a software package designed to facilitate the control and management of various electrophysiology equipment and data acquisition systems. It provides a centralized interface to configure and operate multiple devices simultaneously, enabling efficient and streamlined experimental setups.
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Lab products found in correlation
3 protocols using plexcontrol
1
In Vivo Electrophysiology of Locus Coeruleus
2
Optogenetic Stimulation of Lateral Habenula
3
Mapping Receptive Fields and Orientation Tuning
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Spikes were isolated and sorted online using the software PlexControl (Plexon). We first computed receptive fields and orientation tuning of each cell. After determining an approximate location of the receptive field by manually sweeping a small blinking square (0.2 degrees) across the screen, we mapped receptive fields by computing the spike-triggered average (STA) in response to a random stimulus of size 8 degrees that was centered on the hand-mapped location as described in Hesse and Tsao (12 (link)). A 2-dimensional Gaussian was fitted to the STA to determine the center and size of the receptive field. Subsequently, stimulus position and size were adjusted so that the receptive field was centered on the stimulus and the square contained in the stimulus was larger than the receptive field. Moreover, the preferred orientation of the cell was determined from the sine grating orientation that elicited the highest response, and subsequent stimuli were rotated so that edges centered on the receptive field were presented in the preferred orientation.
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