Samples for DNA extraction were taken weekly from the lab-scale reactors and stored at −20 °C. DNA for amplicon sequencing was extracted using the AllPrep PowerViral DNA/RNA kit (Qiagen, Düsseldorf, Germany) and the Soil DNA Isolation Plus kit (Norgen, Thorold, ON, Canada) in triplicates, according to the manufacturers’ instructions. The hypervariable V3–V4 region of the 16S rRNA gene was used as a marker to analyze the microbial community composition (MCC) in the lab-scale reactors and the mesophilic full-scale WWTP anaerobic digestor. Sequencing of the barcoded amplicons was performed using the Illumina MiSeq platform with V2 chemistry (Illumina Inc., San Diego, CA, USA) according to the manufacturer’s instructions. Primers Pro341f/Pro805r [34 (link)] targeting the domains Archaea and Bacteria, respectively, were used to generate barcoded amplicons for sequencing. Libraries were prepared using the NexteraXT kit (Illumina Inc., San Diego, CA, USA).
Soil dna isolation plus kit
Manufactured by Norgen Biotek
Sourced in Germany
The Soil DNA Isolation Plus kit is a laboratory equipment designed to extract and purify DNA from soil samples. It is a tool used in research and analysis applications that require high-quality genomic DNA from soil.
Automatically generated - may contain errors
2 protocols using soil dna isolation plus kit
1
Microbial Community Analysis in Anaerobic Digesters
2
Genomic DNA Extraction from Microalgae
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To remove the culture medium, 2-10 mL of fresh concentrated culture were centrifuged at 2500g for 5 min at room temperature. The pellet was resuspended in 1 mL of nuclease-free water and re-centrifuged under the same conditions. Genomic DNA was extracted from the pelleted microalgae using the Soil DNA Isolation Plus Kit (Norgen Biotek Corp.) and the PureLink Plant Total DNA Purification Kit (Invitrogen). The total genomic DNA extracted was quantified with the Qubit dsDNA HS Assay Kit (Molecular Probes).
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