Nude mice

0.5x10⁶ DLD1 cells were injected per flank into 6 week-old female Nude mice (Janvier Labs). CAP2 (10mg/kg) complexed with ßcyclodextrin as vehicle, or vehicle alone, were injected every second day intraperitoneally once the tumors were palpable (~5-10mm³). Tumor volumes were measured every other day with a caliper. Animals were sacrificed at the end of the experiment and tumors harvested. All animal procedures were under approved protocols of the Office Cantonal de Veterinaire de Genève. CAP2 from four independent batches (Analyticon Discovery) was dissolved in DMSO and suspended in 45% ßcyclodextrin. The latter was used alone for controls.

NUDE mice were purchased from Janvier Laboratory (France). The protocol for the in vivo metastasis assay on mice has been approved by the Center of ethical committee on animal experiments in Sweden "Centrala försöksdjurnämnden". Ethical Dnr: M129-15. All animals were maintained under specific pathogen-free conditions at the Medicon Village animal facility at Lund University. All animal experiments were performed according to the national and international guidelines of the European Union. All mice were housed under pathogen-free conditions in the animal facility and received autoclaved water and food. Ten control and ten BCL3ANT treated 6-week-old mice were used in this study. The mice were subcutaneously transplanted with 1.10⁶ cells/ml of SK Mel 3 melanoma cell lines. Six days after transplantation, the mice were treated intraperitoneally (IP) with 10 mg/kg BCL3ANT or vehicle control. The vehicle solution used to dissolve BCL3ANT and as a control was composed by 2% DMSO in PBS. The mice were treated every 3 days with BCL3ANT or vehicle and sacrificed after 16 days post transplantation. Tumor volume was measured using CALIPER. Treatment was repeated every third day until day 16. The mice were euthanized by exposure to CO₂ without removing animals from their home cage.

Female 8–10 wk old C57BL/6 and nude mice were purchased from Janvier Laboratories. Xcr1^DTA and OT-I (ovalbumin-specific TCR-transgenic mice) mice obtained from B Malissen’s laboratory have been previously described (Voehringer et al, 2008 (link); Wohn et al, 2020 (link)). Ifngr1^KO mice were purchased from CNRS (UMR 7355). Mice were housed under a standard 12-h:12-h light–dark cycle with ad libitum access to food and nonacid water, 22°C ±1°C, 45–60% humidity, and maintained under specific pathogen-free conditions at an animal facility of the CIML (F13-055-10). For the osteosarcoma murine model, 5-wk-old BALB/cByj mice were purchased from Charles River Laboratories and housed at CRCL animal facilities under the same conditions as mentioned previously. All experiments were conducted in accordance with institutional committees and the French and European guidelines for animal care. The experiments were approved by the Ethical Committee for Animal Experimentation.