Il 17 bv650

Manufactured by BioLegend

Sourced in United States

IL-17-BV650 is a fluorochrome-conjugated antibody used for the detection and analysis of IL-17-expressing cells in flow cytometry applications.

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Lab products found in correlation

2 protocols using il 17 bv650

Multicolor Flow Cytometry Panel

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The following anti-mouse antibodies were used: CD3-Pacific Blue, CD4-PEridinin chlorophyll protein (PerCP)-Cy5.5, CD8-allophycocyanin (APC)-Cy7, CD69-phycoerythrin (PE), CD44-fluorescein isothiocyanate (FITC), CD62L-APC, CD103-PE-Cy7, CD69-FITC, IFN-γ-APC, IFN-γ-BV510, IL-17-PE-Cy7, IL-17-BV650, CD11b-APC-Cy7, CD11c-APC, CD80-FITC, CD86-PerCP-Cy5.5, CD40-PE, CD4-PE, CD4-APC, CD4-FITC, CD3-BV510, and CD3-BV650 from Biolegend, USA.
Alexa Fluor 647 secondary antibody or isotype control IgG-APC was from Abcam.
p-P38, P38, pSTAT3, pSTAT4, and β-tubulin were obtained from Cell Signaling Technology.
I-A(b)_4-17ESAT-6 MHC-II tetramer to analyze the M. tuberculosis-specific CD4⁺ T cell responses was procured from NIH Tetramer Facility, USA (USA).

Kumari A., Pahuja I., Negi K., Ghoshal A., Mukopadhyay S., Agarwal M., Mathew B., Maras J.S., Chaturvedi S., Bhaskar A, & Dwivedi V.P. (2023). Withaferin A Protects against Primary and Recurrent Tuberculosis by Modulating Mycobacterium-Specific Host Immune Responses. Microbiology Spectrum, 11(2), e00583-23.

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Multiparametric Flow Cytometry of Immune Cells

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Following antibodies were used for this study:
Anti-Mouse: CD3-Pacific Blue, CD4-PErCPCy5.5, CD8-APCCy7, CD69-PE, CD44-FITC, CD62L-APC, CD103-PeCy7, CD69-FITC, IFNγ-APC, IFNγ-BV510, IL17-PECy7, IL17-BV650, CD11b-APCCy7, CD11c-APC, CD80-FITC, CD86-PerCPCy5.5, CD40-PE, CD4-PE, CD4-APC and CD4-FITC, CD3-BV510, CD3-BV650 from Biolegend, USA.
Anti-human: CD3-PE, CD4-APCCy7, CD8-Pacific Blue, CD45RO (PerCPCY5.5), CCR7 (PeCy7), CD69 (Alexa Fluor 700) from BD Biosciences.
Anti-human/anti-mouse: STAT3, p-STAT3, STAT4, p-STAT4, AKT, p-AKT, FOXO1, p-FOXO1, NFκB, p-NFκB, BLIMP1 and β-Tubulin from Cell Signaling Technology.

Pahuja I., Negi K., Kumari A., Agarwal M., Mukhopadhyay S., Mathew B., Chaturvedi S., Maras J.S., Bhaskar A, & Dwivedi V.P. (2023). Berberine governs NOTCH3/AKT signaling to enrich lung-resident memory T cells during tuberculosis. PLOS Pathogens, 19(3), e1011165.

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