Hionic fluor cocktail

After administration, urine and feces were collected at 0, 24, 48, 72, 96, 120, 144, and 168 h. The cage was rinsed with distilled water at each sampling period, and the cage rinse was combined as a urine sample. The radioactivity in approximately 0.5 g was measured using the LSC after adding 10 mL of the scintillator Hionic-Fluor cocktail (PerkinElmer, Waltham, MA, USA). Each fecal sample was diluted to 100 mL with distilled water and homogenized. An aliquot of approximately 0.5 g fecal homogenate was dissolved in 2 mL of tissue solubilizer Soluence-350 (PerkinElmer, Waltham, MA, USA) by heating, and radioactivity was measured using an LSC after adding a 10 mL scintillator Hionic-Fluor cocktail. At 96 h after administration, the animals were euthanized by inhalation of carbon dioxide. Each carcass was dissolved in 500 mL of 2 mol/L sodium hydroxide solution (guaranteed grade, Nacalai Tesque, Kyoto, Japan) and 50 mL of toluene (guaranteed grade, Kanto Chemical, Tokyo, Japan) by refluxing with heating. The total volume was finally diluted to 1000 mL with water and homogenized. Radioactivity in 0.5 mL was measured using an LSC after adding a 10 mL scintillator Hionic-Fluor cocktail, and excretion was expressed as a percentage (%) of the administered dose.