Male F344 rats were purchased from CLEA Japan, Inc. In total, 30 rats were divided into a vehicle group, a 10 mg/kg group, and a 100 mg/kg group. AOM (20 mg/kg) was administered intraperitoneally on day 1 and day 4, and rats were given free access to drinking water containing 1% DSS from day 7 to day 13. The dose of resveratrol was determined by weighing rats twice a week, suspending the compound in sterile water, and administering directly to the stomach with a gastric sonde once daily. At 15 weeks after administration, we observed left colon polyps using a thin veterinary endoscope system (AVS Co., Ltd.) in all rats to confirm that the colorectal lesion was successfully produced and avoid meaningless sacrifice. Resveratrol administration was continued for 16 weeks, followed by sacrifice and counting the number of colorectal polyps under a stereomicroscope. Four polyps in each group were excised and frozen for transcriptome analysis. Immediately after sacrifice, blood was collected from the aorta of three animals in each group for analysis.
Detailed methods on PDO culture, compounds, cell viability assay, microarray analysis, bromodeoxyuridine (BrdU) assay, real‐time PCR, western blotting, and statistics are provided in AppendixS1 .
Detailed methods on PDO culture, compounds, cell viability assay, microarray analysis, bromodeoxyuridine (BrdU) assay, real‐time PCR, western blotting, and statistics are provided in Appendix