Serum supernatant was isolated from the inferior vena cava of euthanized mice and cytokines measured using the Cytometric Bead Array (BD Biosciences) with the amount of capture beads, detection reagents, and sample volumes scaled down tenfold from manufacturer’s protocol. Data was collected on an LSRFortessa flow cytometer (BD Biosciences) with FACSDiva v9.0 (BD Biosciences) and analyzed with FlowJo v10 (BD Biosciences). Statistical outliers were removed in GraphPad Prism v9.3 using ROUT method (Q=1%). Cytokine used were mouse TNFα (BD 558299), mouse IL-6 (BD 558301), mouse IL-1α (BD 560157), mouse IL-1β (BD 560232), mouse IL-10 (BD 558300), mouse IL-12/IL-23p40 (BD 560151), and mouse IFN-γ (BD 558296) with Mouse/Rat Soluble Protein Master Buffer Kit (BD 558266).
Mouse il 12 il 23p40
Manufactured by BD
Mouse IL-12/IL-23p40 is a reagent used in laboratory research applications. It is a recombinant protein that represents the p40 subunit common to both the IL-12 and IL-23 cytokines, which are involved in immune system regulation. This product can be used for various in vitro studies, but no further details on intended use are provided.
Automatically generated - may contain errors
Lab products found in correlation
Lsrfortessa flow cytometer, by BD (2 mentions)
Cytometric bead array, by BD (2 mentions)
Facsdiva v9, by BD (2 mentions)
Mouse rat soluble protein master buffer kit, by BD (2 mentions)
Mouse il 1α, by BD (2 mentions)
Mouse il 10, by BD (2 mentions)
Mouse il 1β, by BD (2 mentions)
Mouse ifn γ, by BD (2 mentions)
Mouse il 6, by BD (2 mentions)
Mouse tnf α, by BD (2 mentions)
2 protocols using mouse il 12 il 23p40
1
Cytokine Profiling in Mice Serum
2
Cytokine Profiling from Mouse Serum
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Serum supernatant was isolated from the inferior vena cava of euthanized mice, and cytokines measured using the Cytometric Bead Array (BD Biosciences), with the amount of capture beads, detection reagents, and sample volumes scaled down 10-fold from manufacturer’s protocol. Data were collected on an LSRFortessa flow cytometer (BD Biosciences) with FACSDiva v9.0 (BD Biosciences) and analyzed with FlowJo v10 (BD Biosciences). Statistical outliers were removed in GraphPad Prism v9.3 using ROUT method (Q = 1%). Cytokines used were mouse TNF-α (BD Biosciences 558299), mouse IL-6 (BD Biosciences 558301), mouse IL-1α (BD Biosciences 560157), mouse IL-1β (BD Biosciences 560232), mouse IL-10 (BD Biosciences 558300), mouse IL-12/IL-23p40 (BD Biosciences 560151), and mouse IFN-γ (BD Biosciences 558296) with Mouse/Rat Soluble Protein Master Buffer Kit (BD Biosciences 558266).
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