LDL was isolated from human plasma by density gradient method using Optiprep (60% Iodixanol solution) (Axis-Shield, Norway) by the method of Davies et al [25 (link)]. Human plasma was mixed with Optiprep to obtain 12% iodixanol as a working solution. Optiprep was mixed with PBS to arrive at 9% Iodixanol solution which was dispensed into Optiseal centrifuge tubes (Beckman Coulter, USA) and 3 mL of the iodixanol working solution was carefully under-layered with a syringe and metal cannula. The tubes were placed in NVT 65 near-vertical rotor (Beckman Coulter, USA) and centrifuged at 60,000 rpm for 3 h at 16°C in a Beckman Optima XL-100 ultracentrifuge (Beckman Coulter, USA). LDL separated as interphase was aspirated with syringe and metal cannula and concentrated using SpeedVac Concentrator (Thermo Fisher Scientific, USA).
Beckman optima xl 100
Manufactured by Beckman Coulter
Sourced in United States
The Beckman Optima XL-100 is a high-performance ultracentrifuge capable of reaching speeds up to 100,000 rpm. It is designed for a wide range of applications, including gradient analysis, cell fractionation, and macromolecular separations.
Automatically generated - may contain errors
Lab products found in correlation
Optiseal centrifuge tubes, by Beckman Coulter (1 mentions)
Nvt65 near vertical rotor, by Beckman Coulter (1 mentions)
Speedvac concentrator, by Thermo Fisher Scientific (1 mentions)
Optiprep, by Beckman Coulter (1 mentions)
Ultra clear centrifuge tube, by Beckman Coulter (1 mentions)
Sw41ti rotor, by Beckman Coulter (1 mentions)
2 protocols using beckman optima xl 100
1
LDL Isolation from Human Plasma
2
Isolation of Lipid Droplets from Placenta
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LD were isolated from placental tissue as previously described [22] . Briefly, 5g of placenta were gently rinsed with cold 0.9% NaCl to eliminate blood. Samples were transferred to 12mL buffer (25mM tricine, 250mM sucrose, 0.2mM phenylmethylsulfonyl fluoride, pH 7.6) and homogenized using a T25 digital Ultra-Turrax® (IKA, Staufen, Germany) disperser for 1min followed by homogenization in a tissue grinder Potter-Elvehjem (25 times). Homogenates were centrifuged (3,000g, 10min, 4ºC). Supernatants were transferred to an Ultra-Clear centrifuge tube (Beckman Coulter, CA, USA) and 2mL buffer (20mM HEPES, 100mM KCl, 2mM MgCl 2 , pH 7.4) were loaded on the top of the tissue homogenate. The supernatants were again centrifuged (288,000g, 54min, 4ºC ) in a Beckman Optima XL100 ultracentrifuge equipped with a swinging bucket SW41Ti rotor (Beckman Coulter, CA, USA). After centrifugation, the floating white band containing LD was collected. Analyses were performed by duplicate; one aliquot was used for TG and Chol measures and the other one for FAs quantification.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!