Hkca tlrl hkca

Manufactured by InvivoGen

Sourced in United States

HKCA-#tlrl-hkca is a laboratory equipment product. It serves as a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Zymosan tlrl zyn, by InvivoGen (1 mentions) Stat3 in 1, by MedChemExpress (1 mentions) Sis17, by MedChemExpress (1 mentions) L nmma, by Beyotime (1 mentions) Jsh 23, by MedChemExpress (1 mentions) Trehalose 6 6 dibehenate, by InvivoGen (1 mentions)

2 protocols using hkca tlrl hkca

Immunogenicity of HEWL, HKCA, and Quil-A Adjuvant in Dairy Bulls

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We randomly selected six healthy dairy bulls aged between 4.9–6.7 years and weighing 1000–1100 kg for this study. The six bulls were Holstein breed with similar genetic merit. They were of various genetic backgrounds, according to the pedigree information. Additionally, principal component analysis (PCA) was carried out to analyze the genetic relationships among these animals based on genotypes, showing no significant clustering according to Supplementary Materials File S1: Figure S1. All experimental animals received no veterinary treatment during the course of the experiment. Immunization protocols were conducted from September 2021 to October 2021, and AMIR indicators were evaluated by modified ELISA based on a previous study [6 (link)]. On d0 and d14, the bulls received an intramuscular injection of 0.5 mg of hen egg white lysozyme (HEWL; Sigma L4919, Shanghai, China), 0.5 mg heat-killed Candida albicans (HKCA-#tlrl-hkca, InvivoGen, San Diego, USA), and 0.5 mg of Quil-A adjuvant (#vacquil, InvivoGen, Toulouse, France) dissolved in 1 mL of phosphate-buffered saline (PBS, pH 7.4). On both sides of the neck, an intramuscular injection of 1 mL was given using a single-use needle. The workflow of this study is summarized in Figure 1.

Zhao X., Luo H., Lu H., Ma L., Li Y., Dou J., Zhang J., Ma Y., Li J, & Wang Y. (2023). RNA-Seq Analysis of Peripheral Whole Blood from Dairy Bulls with High and Low Antibody-Mediated Immune Responses—A Preliminary Study. Animals : an Open Access Journal from MDPI, 13(13), 2208.

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Immune Response Modulation Protocols

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Heat-killed C. albicans (HKCA; tlrl-hkca), zymosan (tlrl-zyn) and Trehalose-6,6-dibehenate (TDB; tlrl-tdb) were purchased from Invivogen. Mannan (M3640) were purchased from Sigma-Aldrich. The HDAC11 inhibitors FT895 (HY-112285) and SIS17 (HY-128918), SYK inhibitor R406 (HY-12067), NF-κB inhibitor JSH-23 (HY-13982) and STAT3 inhibitor STAT3-IN-1 (HY-100753) were purchased from MedChemExpress. iNOS inhibitors S-methylisothiourea hemisulfate salt (SMT; S0008) and L-NMMA (S0011) were purchased from Beyotime Biotechnology. The SYK inhibitor R406 (S2194) was purchased from Selleck. The antibodies used in this study shown in Supplementary Table 1.

Wu H., Yin X., Zhao X., Wu Z., Xiao Y., Di Q., Sun P., Tang H., Quan J, & Chen W. (2022). HDAC11 negatively regulates antifungal immunity by inhibiting Nos2 expression via binding with transcriptional repressor STAT3. Redox Biology, 56, 102461.

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