Lgr5 egfp ires creert

Manufactured by Jackson ImmunoResearch

Lgr5-EGFP-IRES-CreERT is a genetically engineered mouse model that expresses enhanced green fluorescent protein (EGFP) and a tamoxifen-inducible Cre recombinase (CreERT) under the control of the Lgr5 gene promoter. Lgr5 is a marker for adult stem cells in various tissues.

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Lab products found in correlation

R26mt mg, by Jackson ImmunoResearch (1 mentions) Tie2 cre, by Jackson ImmunoResearch (1 mentions) R26 tdtomato, by Jackson ImmunoResearch (1 mentions) Lgr5 dtr gfp mice, by Genentech (1 mentions) R26 lacz, by Jackson ImmunoResearch (1 mentions) Cxcl12flox flox, by Jackson ImmunoResearch (1 mentions) Sox2egfp, by Jackson ImmunoResearch (1 mentions) Rosa26lsl yfp mice, by Jackson ImmunoResearch (1 mentions)

2 protocols using lgr5 egfp ires creert

Transgenic Mouse Models in Cancer Research

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Mist1-CreERT mice [36 (link)] and Cxcl12-dsRED mice [37 (link)] were described previously. Cxcr4-EGFP mice were provided from Richard J. Miller (North-western University Medical School). LSL-Kras^G12D and LSL-Trp53^R172H mice were provided by Dr. Kenneth Olive (Columbia University). Cckbr-GFP BAC transgenic mice were purchased from MMRRC (GENSAT project [38 (link)]). Lgr5-DTR-GFP mice were provided by Genentech. Apc^flox/flox mice were obtained from the National Cancer Institute. Lgr5-EGFP-IRES-CreERT, Sox2-EGFP, R26-mTmG, R26-TdTomato, R26-LacZ, Cxcl12^flox/flox, and Tie2-Cre mice were purchased from the Jackson Laboratory. FucciG1 mice were obtained from RIKEN BRC. Cre recombinase was activated by oral administration of tamoxifen (TAM, Sigma, 3 mg/0.2 ml corn oil). All animal studies and procedures were approved by the ethics committees at Columbia University and the University of Tokyo. All mice were bred under specific pathogen free conditions. Comparisons were made with age- and sex- matched control animals.

Sakitani K., Hayakawa Y., Deng H., Ariyama H., Kinoshita H., Konishi M., Ono S., Suzuki N., Ihara S., Niu Z., Kim W., Tanaka T., Liu H., Chen X., Tailor Y., Fox J.G., Konieczny S.F., Onodera H., Sepulveda A.R., Asfaha S., Hirata Y., Worthley D.L., Koike K, & Wang T.C. (2017). CXCR4-expressing Mist1+ progenitors in the gastric antrum contribute to gastric cancer development. Oncotarget, 8(67), 111012-111025.

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Lgr5-Ezh2-Eed Mouse Model Generation

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Lgr5^{EGFP-IRES-CreERT} (Barker et al., 2007 (link)) and ROSA26^lsl-yfp mice were purchased from The Jackson Laboratories, and Ezh2^+/Fl mice (Su et al., 2003 (link)) from the Mutant Mouse Regional Resource Centers. Villin^CreER-T2 (el Marjou et al., 2004 (link)) and Eed^fl/fl mice (Xie et al., 2014 ) were generous gifts from S. Robine (Institut Pasteur, France) and S. Orkin (Boston Children’s Hospital), respectively. Mice were housed in a colony maintained at 23 ± 1°C, 55 ± 15% humidity, and 12 hr light/dark cycles, and handled according to protocols approved by the Animal Care and Use Committee of the Dana-Farber Cancer Institute. Mice were genotyped by PCR before weaning and again at the time of experiments.

Jadhav U., Manieri E., Nalapareddy K., Madha S., Chakrabarti S., Wucherpfennig K., Barefoot M, & Shivdasani R.A. (2020). Replicational Dilution of H3K27me3 in Mammalian Cells and the Role of Poised Promoters. Molecular cell, 78(1), 141-151.e5.

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