4 hydroxypropranolol

Tris buffer, potassium chloride, glycerol and Triton X-100 were sourced from Carl Roth GmbH & Co. KG (Karlsruhe, Germany); UDPGA, (±)-propranolol, (R)-propranolol, and (±)-4-hydroxypropranolol were obtained from Sigma-Aldrich (Taufkirchen, Germany); (±)-5-hydroxypropranolol was purchased from Cayman Chemical Company (Ann Arbor, MI, USA); the NADPH regeneration system was sourced from Promega Corporation (Madison, WI, USA); and 4-methoxypropranolol was synthesized in-house as described previously [30 (link),31 (link),32 (link)]. Ammonium hydrogen carbonate was purchased from VWR International GmbH (Darmstadt, Germany); and phosphate buffered saline (PBS, pH 7.4) was purchased from VWR Chemicals LLC (Solon, OH, USA).

Tris, KCl and Triton X-100 are from Carl Roth GmbH + Co. KG (Karlsruhe, Germany); UDPGA, (±)-propranolol, (R)-propranolol and (±)-4-hydroxypropranolol are from Sigma-Aldrich (St. Louis, Mo, USA); 4-methoxy-1-naphthol and epichlorohydrin are from ThermoFisher GmbH (Kandel, Germany); methylethylketone is from T-E-Klebetechnik (Hannover, Germany); potassium carbonate and NH₄HCO₃ are from VWR International GmbH (Darmstadt, Germany); isopropylamine is from TCI Europe N.V (Haven, Belgium); N-methyl-N-(trimethylsilyl)trifluoracetamid (MSTFA) is from Chemische Fabrik Karl Bucher GmbH (Waldstetten, Germany).