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Nebnext ultra or ultra 2 directional rna kit

Manufactured by New England Biolabs
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The NEBNext Ultra (or Ultra II) Directional RNA Kit is a laboratory equipment product designed for the preparation of directional RNA-seq libraries. It enables the construction of high-quality sequencing libraries from total RNA samples, preserving the original orientation of the RNA transcripts.

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Lab products found in correlation

Tri reagent, by Merck Group (2 mentions) Bioanalyzer 6000 pico chip, by Agilent Technologies (2 mentions) Nebnext poly a mrna magnetic isolation module, by New England Biolabs (2 mentions)

Spelling variants of this product

NEBNext Ultra II (20 citations) NEBNext Ultra II Directional RNA kit (16 citations) NEBNext Ultra II Directional kit (10 citations) Nebnext Ultra Directional RNA kit (7 citations) RNA Ultra Directional kit (7 citations) NEBNext® Ultra RNA kit (5 citations) NEBNext kits (3 citations) Ultra II (3 citations) NEBNext Ultra II RNA (2 citations)

2 protocols using nebnext ultra or ultra 2 directional rna kit

1

Transcriptome Analysis via mRNA-seq

Cited 1 time
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RNA was extracted from cells using TRIreagent (Sigma) following manufacturer’s instructions and RNA integrity assessed using a Bioanalyzer 6000 pico chip (Agilent). mRNA-seq libraries were prepared using the NEBNext Ultra (or Ultra II) Directional RNA Kit (NEB) with the NEBNext Poly(A) mRNA Magnetic Isolation Module and processed for sequencing as previously described in (39 (link)).
Channathodiyil P., May K., Segonds-Pichon A., Smith P.D., Cook S.J, & Houseley J. (2022). Escape from G1 arrest during acute MEK inhibition drives the acquisition of drug resistance. NAR Cancer, 4(4), zcac032.
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2

RNA Extraction and mRNA-Seq Library Prep

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RNA was extracted from cells using TRIreagent (Sigma) following manufacturer's instructions and RNA integrity assessed using a Bioanalyzer 6000 pico chip (Agilent). mRNA-seq libraries were prepared using the NEBNext ultra (or ultra II) Directional RNA kit (NEB) with the NEBNext Poly(A) mRNA Magnetic Isolation Module.
Channathodiyil P., Segonds-Pichon A., Smith P.D., Cook S.J., & Houseley J. (2021). DNA replication during acute MEK inhibition drives acquisition of resistance through amplification of the BRAF oncogene.
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