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2 protocols using folin ciocalteau reagent

1

Antioxidant Compounds Analysis Protocol

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Folin–Ciocalteau reagent and Iron (III) chloride hexahydrate (FeCl3·6H2O) were acquired from Dinâmica (São Paulo, Brazil). Methanol (Merck, Darmstadt, Germany) and formic acid (Panreac, Barcelona, Spain) were HPLC grade. 2,4,6-Tris(2-piridil)-s-triazina (TPTZ), 2,2-difenil-1-picril-hidrazil (DPPH), 6-Hidroxi-2,5,7, 8-tetrametilcromano-2-carboxylic acid (Trolox), 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), potassium persulfate, gallic acid, catechin hydrate, chlorogenic acid, caffeic acid, ellagic acid hydrate, and e quercetin were supplied by Sigma-Aldrich (St Louis, MO, USA). Potassium carbonate and Deionized water were purchased from Synth (Diadema, Brazil) and obtained from a Milli-Q (Millipore, Billerica, MA, USA) purification unit, respectively.
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2

Guava Leaves Antioxidant Potential

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The leaves of the guava plant (Psidium guajava Linn. var. Pomifera) from the seedling bed was kindly donated by the Division of Parks and Conservation Units of the Secretariat for the Environment (Sorocaba, São Paulo, Brazil), ethanol (Synth, São Paulo, Brazil), hydrochloric acid (Neon, São Paulo, Brazil). Folin-Ciocalteau reagent (Dinâmica, São Paulo, Brazil), sodium carbonate (Dinâmica, São Paulo, Brazil), gallic acid (Dinâmica, São Paulo, Brazil). 2,2-diphenyl-1-picrylhydrazyl (Sigma-Aldrich, Steinheim, Germany), methanol (Chemco, São Paulo, Brazil). All reagents used were analytical grade.
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