Anti padpr

The following antibodies were used for immunoblotting assays: anti-pADPr (Rabbit 1:4,000, #528815; Calbiochem), anti-pADPr (Mouse monoclonal, 1:500, 10H - sc-56198; Santa Cruz Biotechnology), anti-B-actin (Mouse monoclonal, 1:5,000, #A5441; Sigma-Aldrich), anti-Tubulin (Mouse monoclonal, 1:20,000, B512; Sigma-Aldrich) and anti-GFP (Mouse monoclonal, #632380, 1:4,000; BD Biosciences). Western blotting was performed using the detection kit from Amersham/GE Healthcare (#RPN2106), according to the manufacturer’s instructions.

The following commercially available antibodies were used: anti-Repo (1:250, DSHB, 8D12), anti-Caspase-3 (1:250 for Immunocytochemistry of fly brain, 1:1000 for Western blot analysis, Cell Signaling Technology, 9665), anti-Cleaved Caspase-3 (1:1000, Santa Cruz, 9661), anti-H2AvD (1:50, Rockland, 600-401-914), anti-p53(E-5) (1:50, Santa Cruz, sc-74573), p53(DO-1) (1:1000, Santa Cruz, sc-126), anti-Drosophila Nmnat (1:3000), anti-NMNAT1 (1:1000, Abcam, ab45548), anti-NMNAT1 (1:1000, Santa Cruz, 271557), anti-NMNAT2 (1:500, Abcam, ab56980), anti-PARP1 (1:1000, Santa Cruz, sc-8007), anti-pADPr (1:1000, Santa Cruz, sc-56198), anti-SIRT1 (1:1000, Cell Signaling Technology, 2492), anti-acetyl-p53 (1:1000, Cell Signaling Technology, 2525), anti-β-actin (1:10,000, Sigma-Aldrich, A1978), and anti-tubulin (1:300, Abcam, ab15246). The secondary antibodies conjugated to Alexa 488/546/647 (1:250, Invitrogen), or near-infrared (IR) dye 700/800 (1:5000, LI-COR Biosciences). HRP-anti-mouse and HRP-anti-rabbit (1:5000, Thermo Fisher Scientific).