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Cd11b alexa 647 clone m1 70

Manufactured by Thermo Fisher Scientific
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CD11b-Alexa 647 (clone M1/70) is a fluorescently-labeled antibody that binds to the CD11b antigen expressed on the surface of certain immune cells, such as monocytes, macrophages, and granulocytes. The antibody is conjugated to the Alexa Fluor 647 fluorescent dye, which can be detected using flow cytometry or other fluorescence-based analytical techniques.

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Lab products found in correlation

Oct medium, by Electron Microscopy Sciences (2 mentions) Vgf peptide antibody, by Thermo Fisher Scientific (2 mentions) Alexa 568 anti rabbit, by Thermo Fisher Scientific (2 mentions) Leica cryostat, by Leica Microsystems (2 mentions) Inverted sp5 confocal microscope, by Leica Microsystems (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by PerkinElmer (2 mentions)

2 protocols using cd11b alexa 647 clone m1 70

1

Immunohistochemical Analysis of Ear Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ears were fixed in periodate-lysine-paraformaldehyde overnight as reported 49 (link), cryoprotected in 15% sucrose, embedded in OCT medium (Electron Microscopy Sciences) and frozen in dry-ice cooled isopentane. Eighteen-micron cross-sections were cut on a Leica cryostat (Leica Microsystems), blocked with 5% goat or donkey serum, then stained with a combination of DAPI (Perkin Elmer), CD11b-Alexa 647 (clone M1/70, eBioscience), and VGF peptide antibody followed by anti-rabbit Alexa 568 (Invitrogen). Images were acquired on an inverted SP5 confocal microscope (Leica Microsystems) using identical PMT (photomultiplier tube) and laser settings. Lesion depth was scored manually using Imaris software.
Beerli C., Yakimovich A., Kilcher S., Reynoso G.V., Fläschner G., Müller D.J., Hickman H.D, & Mercer J. (2018). Vaccinia virus hijacks EGFR signalling to enhance virus spread through rapid and directed infected cell motility. Nature microbiology, 4(2), 216-225.
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2

Immunohistochemical Analysis of Ear Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ears were fixed in periodate-lysine-paraformaldehyde overnight as reported 49 (link), cryoprotected in 15% sucrose, embedded in OCT medium (Electron Microscopy Sciences) and frozen in dry-ice cooled isopentane. Eighteen-micron cross-sections were cut on a Leica cryostat (Leica Microsystems), blocked with 5% goat or donkey serum, then stained with a combination of DAPI (Perkin Elmer), CD11b-Alexa 647 (clone M1/70, eBioscience), and VGF peptide antibody followed by anti-rabbit Alexa 568 (Invitrogen). Images were acquired on an inverted SP5 confocal microscope (Leica Microsystems) using identical PMT (photomultiplier tube) and laser settings. Lesion depth was scored manually using Imaris software.
Beerli C., Yakimovich A., Kilcher S., Reynoso G.V., Fläschner G., Müller D.J., Hickman H.D, & Mercer J. (2018). Vaccinia virus hijacks EGFR signalling to enhance virus spread through rapid and directed infected cell motility. Nature microbiology, 4(2), 216-225.
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