Inveon research workplace software version 4.2 irw

Data analysis of the SPECT/CT fused images were performed with the INVEON Research Workplace software, version 4.2 (IRW; Siemens Healthcare, Ballerup, Denmark). Procedure is described in a previous model [13] .
μCT Volumetric measures of hindlimbs were calculated from acquired CT images. To standardize the analysis the distal tibiofibular joint was chosen as the upper volumetric boundary limit [19] . The volume of the hindlimb distally to this joint was then calculated using simple thresholding techniques. All voxels within the Hounsfield interval of -500 Hounsfield units and 4000 Hounsfield units were included in the hindlimb volume; see Figure 6. Figure 6. μCT scan showing the volumetric measures of both hindlimbs from mouse number 13 (SVF-group) from week 2. The distal tibiofibular joint was chosen as the upper volumetric boundary limit and can be seen just superior to the highlighted areas. The volume of the hindlimb distally to this joint was then calculated using simple thresholding techniques. All voxels within the Hounsfield interval of -500 Hounsfield Units and 4000 Hounsfield Units were included in the hindlimb volume. μCT: micro-computed tomography; SVF: stromal vascular fraction.

Data analysis of the SPECT/CT fused images was performed with INVEON Research Workplace software, version 4.2 (IRW, Siemens Healthcare, Ballerup, Denmark). The lymphatic clearance of the operated-and non-operated limb was calculated from lymphoscintigraphy scans made 45 min and 4 h after tracer injection. Volumes of interest were defined around the injection site. The activity decline from the injection site was presumed to follow a monoexponential function e -kt . The removal rate was calculated as 100%k (%/min) [19] .