α phospho p38 t180 y182

Manufactured by Cell Signaling Technology

The α-phospho-p38 (T180/Y182) is a laboratory reagent that can be used to detect the phosphorylation of p38 mitogen-activated protein kinase (MAPK) at threonine 180 and tyrosine 182 residues. This product is intended for research use only.

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Lab products found in correlation

α hog1 yc 20, by Santa Cruz Biotechnology (2 mentions) α ha 16b12, by Fortrea (1 mentions) α myc, by Santa Cruz Biotechnology (1 mentions) Mouse anti goat, by Santa Cruz Biotechnology (1 mentions) α mpk1 yc 20, by Santa Cruz Biotechnology (1 mentions) α phospho p44 42 mapk thr202 tyr204, by Cell Signaling Technology (1 mentions) αha 16b12, by BioLegend (1 mentions)

Close spelling variants of this product

Phospho-p38

2 protocols using α phospho p38 t180 y182

Protein Detection via Immunoblotting

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Proteins were separated by SDS–PAGE (7.5% gels) followed by immunoblot analysis using mouse monoclonal α-Myc antibody (9E10; Santa Cruz), α-HA (16B12; Covance), α-GST (B-14; Santa Cruz), α-tetra His (Qiagen), or goat polyclonal α-Hog1 (yC-20; Santa Cruz) at a dilution of 1:10,000. Rabbit polyclonal α-phospho-p38 (T180/Y182, Cell Signaling) was used at a dilution of 1:2000 to detect phosphorylated Hog1. Secondary goat anti-mouse (Amersham) and donkey anti-goat (Santa Cruz) antibodies were used at a dilution of 1:10,000. Secondary donkey anti-rabbit (Amersham) was used at a dilution of 1:2000. All results involving immunoblot analyses were replicated at least once, and representative blots are shown.

Lee J, & Levin D.E. (2018). Intracellular mechanism by which arsenite activates the yeast stress MAPK Hog1. Molecular Biology of the Cell, 29(15), 1904-1915.

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Immunoblot Analysis of Yeast Proteins

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Proteins were separated by SDS-PAGE (10% gels) followed by immunoblot analysis using mouse monoclonal antibodies α-Myc (9E10; Santa Cruz), α-HA (16B12; BioLegend), α-Carboxypeptidase Y (CPY; 10A5B5; Abcam), or goat polyclonal α-Mpk1 (yC-20; Santa Cruz) at a dilution of 1:10,000. Rabbit polyclonal α-phospho-p44/42 MAPK (Thr202/Tyr204; Cell Signaling), was used at a dilution of 1:2,000 to detect phosphorylated Mpk1. Goat polyclonal α-Hog1 (yC-20; Santa Cruz) at a dilution of 1:10,000, or rabbit polyclonal α-phospho-p38 (T180/Y182, Cell Signaling) at a dilution of 1:2,000 were used to detect Hog1 and phosphorylated Hog1, respectively. Secondary goat anti-mouse (Jackson ImmunoResearch Labs), donkey anti-rabbit (GE Healthcare), or mouse anti-goat (Santa Cruz) antibodies were used at a dilution of 1:10,000. All results involving immunoblot analyses were replicated at least once and representative blots are shown.

Laz E.V., Lee J, & Levin D.E. (2019). Crosstalk between S. cerevisiae SAPKs Hog1 and Mpk1 is mediated by glycerol accumulation. Fungal biology, 124(5), 361-367.

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