Citrate concentration was analysed by ion chromatography (IC) with suppressed conductivity detection (ICS-5000+ ion chromatographic system; AS-AP autosampler; ATC-3 trap column installed between pump and injection valve; IonPac 4x250 mm AS11-HC ion-exchange analytical column coupled with a AG11-HC 4mm guard column; anion self-regenerating suppressor ASRS 300 (4mm) in auto suppression recycle mode; Dionex, Thermo Fischer Scientific, USA). Ten µL sample was injected. Separation was performed with NaOH in laboratory grade I water as eluent, with a flow rate of 1.5 mL min H 2 O. An external standard of citrate (Sigma Aldrich, citric acid, ACS reagent, ≥ 99.5%) was used for identification (retention time) and quantification after the construction of a calibration curve (R 2 =0.99).
Atc 3 trap column
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ATC-3 trap column is a laboratory equipment designed for the purification and concentration of analytes in liquid samples. It utilizes a solid-phase extraction (SPE) technique to selectively retain and separate target compounds from the sample matrix. The core function of the ATC-3 trap column is to facilitate the sample preparation process, enabling the user to extract, purify, and concentrate analytes of interest prior to further analysis.
Automatically generated - may contain errors
Lab products found in correlation
As ap autosampler, by Thermo Fisher Scientific (2 mentions)
Ionpac 4x250 mm as11 hc ion exchange analytical column, by Thermo Fisher Scientific (2 mentions)
Ag11 hc 4mm guard column, by Thermo Fisher Scientific (2 mentions)
Asrs 300 4mm in, by Thermo Fisher Scientific (2 mentions)
Ics 5000 ion, by Thermo Fisher Scientific (2 mentions)
Citric acid, by Merck Group (2 mentions)
2 protocols using atc 3 trap column
1
Citrate Quantification by Ion Chromatography
2
Citrate Quantification by Ion Chromatography
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Citrate concentration was analysed by ion chromatography (IC) with suppressed conductivity detection (ICS-5000+ ion chromatographic system; AS-AP autosampler; ATC-3 trap column installed between pump and injection valve; IonPac 4x250 mm AS11-HC ion-exchange analytical column coupled with a AG11-HC 4mm guard column; anion self-regenerating suppressor ASRS 300 (4mm) in auto suppression recycle mode; Dionex, Thermo Fischer Scientific, USA). Ten µL sample was injected. Separation was performed with NaOH in laboratory grade I water as eluent, with a flow rate of 1.5 mL min -1 and constant temperature of 30°C, with the following gradient: E1 (NaOH 1mM) and E2 (NaOH 60mM); 0-15 min 20% E1 80% H2O, 15-25 min 15% E2 85% H2O, 25-35 min 30% E2 70% H2O, 35-45 min 60% E2 40% H2O. An external standard of citrate (Sigma Aldrich, citric acid, ACS reagent, ≥ 99.5%) was used for identification (retention time) and quantification after the construction of a calibration curve (R 2 =0.99). Seven standards with the following concentrations were used: 2.60. 5.20, 26, 52, 130, 260, 520 µmol L -1 . Limit of quantification was 0.014 µmol L -1 .
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