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R3.5 1 holey copper grid

Manufactured by Quantifoil
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Sourced in Germany

The Quantifoil R3.5/1 holey copper grid is a specimen support designed for use in transmission electron microscopy (TEM). It features a regular array of holes in a thin carbon film supported by a copper mesh. The core function of this product is to provide a stable platform for holding and positioning samples for TEM analysis.

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Lab products found in correlation

Gatan ultrascan usc 4000, by Ametek (2 mentions) Polara electron microscope, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Quantifoil R3.5/1 (9 citations) Copper grids (2 citations)

2 protocols using r3.5 1 holey copper grid

1

Cryo-EM of Streptococcus pneumoniae

Check if the same lab product or an alternative is used in the 5 most similar protocols
For cryo-EM, 4 mL of S. pneumoniae R800 cells in exponential growth were loaded onto a Quantifoil R3.5/1 holey copper grid (Quantifoil Micro Tools GmbH, Germany). Vitrification of the sample was performed manually (blotting the sample for 1-2 s and plunging rapidly the grid into liquid ethane cooled with liquid nitrogen). The frozen grid was transferred into a 300 kV POLARA electron microscope (FEI). The images were taken under high dose conditions at 300 kV (80 e -3A ˚À2 ), with a magnification of 12,000 and a defocus between 4 and 5 mm using a CCD camera (GATAN ultrascan USC 4000).
Trouve J., Zapun A., Arthaud C., Durmort C., Guilmi A.M.D., Soederstroem B., Grangeasse C., Bourgeois D., Wong Y., & Morlot C. (2020). Nanoscale Dynamics of Peptidoglycan Assembly During the Cell Cycle of <i>Streptococcus pneumoniae</i>.
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2

Cryo-EM of Streptococcus pneumoniae

Check if the same lab product or an alternative is used in the 5 most similar protocols
For cryo-EM, 4 mL of S. pneumoniae R800 cells in exponential growth were loaded onto a Quantifoil R3.5/1 holey copper grid (Quantifoil Micro Tools GmbH, Germany). Vitrification of the sample was performed manually (blotting the sample for 1-2 s and plunging rapidly the grid into liquid ethane cooled with liquid nitrogen). The frozen grid was transferred into a 300 kV POLARA electron microscope (FEI). The images were taken under high dose conditions at 300 kV (80 e -3A ˚À2 ), with a magnification of 12,000 and a defocus between 4 and 5 mm using a CCD camera (GATAN ultrascan USC 4000).
Trouve J., Zapun A., Arthaud C., Durmort C., Di Guilmi A.M., Söderström B., Pelletier A., Grangeasse C., Bourgeois D., Wong Y.S, & Morlot C. (2021). Nanoscale dynamics of peptidoglycan assembly during the cell cycle of Streptococcus pneumoniae. Current biology : CB, 31(13).
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