D18 1 d5 c18 0 gm3

Manufactured by Avanti Polar Lipids

D18:1-d5-C18:0 GM3 is a synthetic glycosphingolipid compound. It is a form of ganglioside GM3 containing a deuterium-labeled oleic acid (18:1) and a stearic acid (18:0) moiety. This compound is typically used as a standard or internal reference in analytical studies.

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Lab products found in correlation

3200 q trap system, by AB Sciex (2 mentions) Prominence uflc system, by Shimadzu (2 mentions) C17 0 sm, by Avanti Polar Lipids (2 mentions) C17 0 ceramide cer, by Avanti Polar Lipids (2 mentions) C17 0 lactosylceramide, by Avanti Polar Lipids (2 mentions) C17 0 glucosylceramide, by Avanti Polar Lipids (2 mentions) C17 0 gb3, by Avanti Polar Lipids (2 mentions)

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C18:0 (5 citations)

2 protocols using d18 1 d5 c18 0 gm3

Lipidomic Analysis of Cultured Cells

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Cells were seeded in a 6-cm dish at a density of 0.5 × 10⁶ cells/dish in 5 ml of culture medium. After 24 h, cells were washed twice with PBS and harvested. A total of 1 nmol each of the internal standards C17:0 SM, C17:0 ceramide (Cer), C17:0 lactosylceramide, C17:0 glucosylceramide, C17:0 Gb3, and d18:1-d5-C18:0 GM3 (Avanti Polar Lipids) were then added for LC-MS/MS analysis. The amount of protein was determined and lipids were extracted from the cells. Sphingolipids were analyzed by an LC-MS system that consisted of a Prominence UFLC system (Shimadzu) coupled to a 3200 QTRAP System (SCIEX) as described previously (27 ).

Tamura N., Sakai S., Martorell L., Colomé R., Mizuike A., Goto A., Ortigoza-Escobar J.D, & Hanada K. (2021). Intellectual-disability-associated mutations in the ceramide transport protein gene CERT1 lead to aberrant function and subcellular distribution. The Journal of Biological Chemistry, 297(5), 101338.

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Sphingolipid Quantification by LC-MS/MS

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Cells were seeded in a 6-cm dish at a density of 0.5 × 10⁶ cells/dish in 5 mL of culture medium. After 24 h, cells were washed twice with PBS and harvested. The amount of protein was determined and lipids were extracted from the cells. A total of 1 nmol each of the internal standards C17:0 SM, C17:0 ceramide (Cer), C17:0 lactosylceramide, C17:0 glucosylceramide, C17:0 Gb3, and d18:1-d5-C18:0 GM3 (Avanti Polar Lipids, Inc) were then added for quantification. Sphingolipids were analyzed by an LC-MS/MS system that consisted of a Prominence UFLC system (Shimadzu Corporation) coupled to a 3200 QTRAP System (SCIEX) as described previously (Nakao et al., 2019 (link)).

Goto A., Sakai S., Mizuike A., Yamaji T, & Hanada K. (2022). Compartmentalization of casein kinase 1 γ CSNK1G controls the intracellular trafficking of ceramide. iScience, 25(7), 104624.

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