WP aerogel particles were prepared as previously described. 6 Briefly, WP aqueous solutions (20%, w/w) were adjusted at pH 5.7 and gelled at 85 °C for 15 min in sealed 50 mL plastic tubes. The obtained hydrogel was cooled and homogenized using a high-speed mixer at 13 000 rpm for 3 min (Polytron PT-MR3000, Kinematica AG, Littau, Switzerland). The hydrogel particles were then dispersed in ethanol (0.1 g mL -1 ), homogenized and collected by centrifugation at 13 000g for 10 min at 4 °C (Avanti J-25, Beckman, Palo Alto, CA, USA). This procedure was repeated twice to completely remove water. The ethanol was then removed using a supercritical CO 2 -drying plant at 60 °C and 120 bar. The obtained dried aerogel particles were refined by grinding for 1 min using a domestic grinder (MC3001, Moulinex, Milan, Italy). The powder was then dispersed into oil (0.1 g mL -1 ) and homogenized at 13 000 rpm for 3 min. The oil-absorbed particles were collected by centrifugation, as previously described. The collected particles were re-dispersed in oil (0.1 g mL -1 ) and centrifuged, obtaining oleogels presenting 80% (w/w) oil content, whose complete characterization has been previously reported. 6,7
Polytron pt mr3000
Manufactured by Kinematica
Sourced in Switzerland
The Polytron PT-MR3000 is a high-performance rotor-stator homogenizer designed for effective dispersing, emulsifying, and high-shear mixing applications. It features a robust and powerful motor with adjustable speed control, allowing for flexible operation across a wide range of viscosities and sample volumes.
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Lab products found in correlation
5 protocols using polytron pt mr3000
1
Preparation of Aerogel Particles from Whey Protein
2
Antifungal Compound Effects on Candida
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Candida albicans cells grown overnight at 37 °C in the presence of isolated antifungal active compound (50 μg/ml) were harvested by centrifugation and then suspended in homogenizing buffer (1 mmol/L phenylmethylsulphonyle fluoride, 250 mmol/L sucrose, 10 mmol/L Tris–HCl, pH 7.5). The control sample was also prepared and did not contain the active compound. The cells were then mechanically disrupted at 4 °C using a soniprobe (Polytron PT-MR 3000, Kinematica AG, Littau, Switzerland). The homogenate was collected and centrifuged at 20217 × g-force for 1 hour at 4 °C. The supernatant was collected and investigated for its protein concentration and antioxidant enzyme activity.
3
Ionic Strength and Protein Isolate Effects
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Aqueous solutions presenting different ionic strength (IS), 0.6 and 1.5 M, were prepared by adding NaCl (Sigma Aldrich, Milan, Italy) in deionised water (System advantage A10®, Millipore S.A.S, Molsheim, France). Deionised water without the addition of NaCl was considered to have an IS equal to 0.0 M. Aqueous solutions were added with 10, 15, or 20% (w/w) of soy (SPI) or pea (PPI) protein isolates (Myprotein, Manchester, England). The suspensions were subjected to high shear mixing at 1120× g for 1 min (Polytron PT-MR3000, Kinematica AG, Littau, Switzerland), and pH was adjusted to 3.0, 4.5, and 7.0 by adding 1 M NaOH or HCl.
4
Developing Lipid Carrier Formulations
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Table 2 lists the oral and topical LC formulations used in this study. These formulations were designed based on changing the concentration of MGE in the formulations and entrapped with drugs with different physiochemical properties. The mixture was dispersed using a homogenizer (Polytron PT-MR 3000; Kinematica Inc., Littau, Switzerland) . The dry powder LC formulations were prepared using a freeze dryer (Labcono FZ-18; Asahi Life Science Co. Ltd., Tokorozawa, Saitama, Japan) .
5
Aerogel Particles Preparation Protocol
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WP aerogel particles were prepared as described by Plazzotta et al. [26 (link)]. Briefly, WP isolate aqueous solutions (20% w/w) were adjusted at pH 5.7 and gelled at 85 °C for 15 min. The obtained hydrogel was cooled and homogenized by using a high-speed mixer at 13,000 rpm for 3 min (Polytron PT-MR3000, Kinematica AG, Littau, Switzerland). The hydrogel was then dispersed in ethanol (0.1 g/mL), homogenized and collected by centrifugation at 13,000 g for 10 min at 4 °C (Avanti J-25, Beckman, Palo Alto, CA, USA). This procedure was repeated twice to completely remove water. The alcolgel particles were dried at a temperature of 60 °C, pressure of 120 bar, under a continuous flow of CO2 (flow rate = 120–160 g/min) through the autoclave until complete extraction of EtOH was achieved after 6 h. The dried particles were ground 1 min using a domestic grinder (MC3001, Moulinex, Milan, Italy) and dispersed into oil (0.1 g/mL), homogenized and collected by centrifugation, as previously described. This procedure was repeated twice, obtaining the oleogels.
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