Oci ly7

The 16 DLBCL cell lines (DOHH2, HT, OCI-LY19, DB, OCI-LY1, SUDHL-4, SUDHL-5, SUDHL-10, NUDHL-1, OCI-LY7, WSU-DLCL2, SUDHL-6, NUDUL-1, U2932, OCI-LY3, and RI-1) were purchased from the American Type Culture Collection or from DSMZ (Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Germany). They were grown in RPMI-1640 Glutamax medium (Gibco, Invitrogen, Cergy Pontoise, France), supplemented with 10% fetal bovine serum (FBS) (PAA laboratory GmbH, Pasching, Austria) (U2932, SUDHL-4, HT, DOHH2, SUDHL-10, RI-1, and WSU-DLCL2 cells), 20% FBS (OCI-LY3, DB, SUDHL-5, NUDHL-1, and SUDHL-6 cells), or 15% FBS (NUDUL-1 cells). OCI-LY1, and OCI-LY7 cells were cultured in IMDM Glutamax (Gibco, Invitrogen, Cergy Pontoise, France), supplemented with 20% FBS, and OCI-LY19 cells in MEM alpha modified Glutamax (Gibco, Invitrogen, Cergy Pontoise, France) with 20% FBS. Cultures were maintained at 37 °C in a humidified atmosphere with 5% CO₂. Contamination by Mycoplasma species was regularly monitored.

The cell lines of DLBCL including OCI-LY7, TMD8 and 293T were purchased from the American Type Culture Collection (ATCC). We packaged lenti-virus by 293T cell. The sgRNA targeting NLE1 or negative control as follow: NLE1-sg1: TGAGCCGATACAACCTCGTG; NLE1-sg3: ACTGACTATGCCCTGCGCAC; Nontarget-sg: ACGGAGGCTAAGCGTCGCAA. After knocking out the NLE1, we tested the proliferation by the EdU staining.

The cell lines OCI-LY7, OCI-LY3 and HEK293T were obtained from American Type Culture Collection (ATCC). OCI-LY7 is germinal center B-cell (GCB)-subtype cell line; OCI-LY3 is an activated B-cell (ABC)-subtype cell line; and HEK293T is an embryonic kidney cell line. OCI-LY7 was maintained in complete Iscove’s modified essential medium (IMDM; GIBCO, Carlsbad, CA, USA) with 2-mercaptoethanol (1:10000) and 10% fetal bovine serum (GIBCO). OCI-LY3 was cultured in IMDM with 20% fetal bovine serum. HEK293T cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM; GIBCO) supplemented with 10% fetal bovine serum. All of the cell lines were supplemented with 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA). All of the cell cultures were maintained at 37°C under 5% CO₂ and 95% air.

Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA). GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA). DLBCL cells were grown in RPMI 1640 with 10% FBS and 0.05 mg/mL gentamycin (Schering-Plough Europe, Brussels, Belgium). 293T cells were grown in DMEM (Thermo Fisher Scientific) containing 10% FBS and 1% pen/strep. Cells were cultured at 37 °C in 5% CO₂.

The human lymphoma cell lines (Mino, Maver-1, Jeko-1, Z-138, OCI-Ly7, OCI-Ly10, SU-DHL-6, SU-DHL-8, U2932, Daudi, Namalwa, Raji, and Ramos) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and DSMZ (Braunschweig, Germany). Cells were cultured in RPMI1640 medium containing 10% foetal bovine serum, 100 U/mL penicillin, and 100 mg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37 °C in an atmosphere of 5% CO₂. OCI-Ly7 and OCI-Ly10 were cultured in IMDM. Tests for mycoplasma contamination were negative. Ibrutinib and venetocalx were purchased from Selleck Chemicals (Houston, TX, USA).

Human lymphoblastoid B cell (GM12878), human renal epithelial cells (293 T) and DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC, USA). The 293 T cells were maintained in DMEM with 10%(v/v) FBS. The GM12878 cells were cultured in Roswell Park Memorial Institute 1640 medium (RPMI 1640; Gibco) supplemented with 15% fetal bovine serum (FBS; Gibco) and 1% penicillin–streptomycin (Gibco) at 37℃ with 5% CO2.