The human GBM cell lines (U87 and U118) and normal stroma cell line (Hs5) were obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific, MA, USA) with the addition of 10% fetal bovine serum (FBS, Thermo Fisher Scientific) and 1% penicillin and streptomycin (Thermo Fisher Scientific) at 37 °C in a humidified atmosphere of 5% CO2 inside the incubator, INCOMED153 (Memmert GmbH & Co, Germany) .
Incomed153
Manufactured by Memmert
Sourced in Germany
The INCOMED153 is a compact, high-precision incubator from Memmert, designed for temperature-controlled incubation of samples in a laboratory setting. It features a stainless-steel interior and an intuitive control panel for accurate temperature regulation.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (2 mentions)
Atcc htb 14, by American Type Culture Collection (2 mentions)
Normal hs 5 cells, by American Type Culture Collection (1 mentions)
Atcc crl 11882, by American Type Culture Collection (1 mentions)
Ts2r fl, by Nikon (1 mentions)
Fgmtm 2 singlequots supplements, by Lonza (1 mentions)
Fbmtm basal medium, by Lonza (1 mentions)
5 protocols using incomed153
1
Culturing Human Glioblastoma Cell Lines
2
Glioblastoma and Normal Cell Culture
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Human glioblastoma U87 and normal Hs-5 cells were obtained from the American Type Culture Collection (Manassas, VA, USA) and maintained in Dulbecco’s Modified Eagle’s culture medium supplemented with 10% fetal bovine serum (Life Technologies, Houston, TX, USA) and 1% antibiotic-antimycotic mixture containing penicillin and streptomycin (Life Technologies). Culture was performed at 37 °C under 5% CO2 and at 95% humidity in an INCOMED153 (Memmert GmbH & Co., Germany).
3
Glioblastoma and Bone Marrow Stromal Cell Lines
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Human glioblastoma U87 MG (ATCC® HTB-14™) and bone marrow stromal Hs5 (ATCC® CRL-11882™) cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA) and maintained in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 10% fetal bovine serum (Life Technologies, Houston, TX, USA) and 1% antibiotic-antimycotic mixture containing penicillin and streptomycin (Life Technologies, Houston, TX, USA). Cultures were maintained at 37 °C under 5% CO2 and 95% humidity in an INCOMED153 (Memmert GmbH & Co. KG, Schwabach, Germany).
4
Glioblastoma and Bone Marrow Stromal Cell Culture
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For cell culturing [27 (link)], the human glioblastoma U87 MG (ATCC® HTB-14™) and bone marrow stromal Hs5 (ATCC® CRL-11882™) cell lines were used from the American Type Culture Collection (Manassas, VA, USA). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (Life Technologies, Houston, TX, USA) and 1% antibiotic-antimycotic mixture containing penicillin and streptomycin (Life Technologies, Houston, TX, USA) at 37 °C under 5% CO2 and 95% humidity in an INCOMED153 (Memmert GmbH & Co. KG, Schwabach, Germany).
5
Primary Human Skin Fibroblast Culture
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Primary human skin fibroblasts (LONZA, CC-2511 NHDF) were cultured in the FGM-2 Fibroblast Bullet Kit medium with FBMTM Basal Medium (CC-3131, Lonza), FGMTM-2 SingleQuotsTM supplements (CC-4126, Lonza), and a mixture of antibiotics (Pen/Strep/Fungizone, 17-745E, Lonza) in a tissue culture incubator (INCOmed 153, Memmert) in an atmosphere of 5% CO2 at 37°C and 90% humidity. For experiments, cultured cells were thawed between passages 4 and 7, and cell growth was monitored under an inverted microscope (Nikon TS2RFL). The cell density at seeding was approximately 4 x 104 cells/well on the 6-well culture plate. The volume of medium used in each well was 2.0 mL and was mentioned the next day.
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