Crl 1628

The cell lines SCC25, derived from a SCC of the tongue, and FaDu derived from a SCC of the hypopharynx, were used in this study. They were obtained directly from American Type Culture Collection (catalog numbers CRL-1628 and HTB-43). The cell lines were grown in a Dulbecco’s Modified Eagle’s medium/Nutrient Mixture F-12 Ham (DMEM/F12) supplemented with 10% fetal bovine serum in a humidified atmosphere of 5% CO₂ and 95% air at 37 °C.

Human squamous carcinoma cell lines of the oral tongue (SCC-4, SCC-9, SCC-25), hypopharynx (FaDu), and of a metastasis from the pharynx (Detroit 562) were purchased from the American Type Culture Collection (Manassas, VA, USA) (CRL-1624, CRL-1629, CRL-1628, HTB-43, and CCL-138). The UM-SCC-22A (hypopharynx) cell line was purchased from EMD Millipore Corporation (Burlington, MA, USA) (SCC076). The cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco™, 11995-065; Waltham, MA, USA) supplemented with 10% fetal bovine serum (Wisent, 088150; Saint-Jean-Baptiste, QU, Canada), 1% penicillin/streptomycin (Wisent, 450-201-EL; Saint-Jean-Baptiste, QU, Canada), and 1 times non-essential amino acids (Gibco™, 11140-050; Waltham, MA, USA), and they were used before the tenth passage from the first thaw after reception. All of the cell lines are HPV-negative.

The Burkitt’s Lymphoma cell line Daudi (CCL-213™) expressing CD38 and CD20 and head and neck squamous cell carcinoma SCC25 (CRL-1628™) cell line expressing EGFR were obtained from the American Type Culture Collection (ATCC, USA). Daudi cells were cultivated in RPMI 1640 medium (ATCC modification) (Gibco, Thermo Fisher Scientific, USA) supplemented with 10% heat-inactivated fetal bovine serum (Sigma Aldrich, USA) and 100 U/ml penicillin + 100 mg/ml streptomycin (Gibco, Thermo Fisher Scientific, USA), at 37°C in a humidified atmosphere containing 5% CO_2. SCC25 cells were cultivated in a 1:1 mixture of Dulbecco’s modified Eagle’s medium (DMEM) and Ham’s F12 medium (Gibco, Thermo Fisher Scientific, USA) supplemented with 400 ng/ml hydrocortisone (Sigma Aldrich, USA), 10% heat-inactivated fetal bovine serum and 100 U/ml penicillin + 100 mg/ml streptomycin at 37°C in a humidified atmosphere containing 5% CO₂. K562 (CCL-243™) CML lymphoblast cell line was obtained from ATCC. K562 were cultivated in DMEM supplemented with 10% heat-inactivated fetal bovine serum at 37°C in a humidified atmosphere containing 5% CO₂. The K562 cell line is widely used as a highly sensitive in vitro target for the natural killer assay.