Infinity 1 camera microscope

Following sacrifice, the liver, kidneys, heart, and lungs were removed immediately, weighed (absolute organ weight), fixed in formalin 10% for 3±1 days, embedded in paraffin wax, sectioned into 3–4 µm sections, and stained with hematoxylin and eosin. The colored sections were observed using optical microscopy (Optika Microscopes, Italy) and captured by an Infinity 1 camera microscope under objective 40. The relative organ weight (ROW) of each animal was calculated as follows [53 ]: $$\mathrm{Relative}\mathrm{Organ}\mathrm{Weight}(\%)=\frac{\mathrm{Organ}\mathrm{weight}\left(\mathrm{g}\right)}{\mathrm{Body}\mathrm{weight}\left(\mathrm{g}\right)}\times 100$$
Histopathological study includes observing tissue integrity and looking for injuries, such as degeneration, necrosis, apoptosis, and leukocyte infiltration, which could be a symptom of toxicity.

Macroscopic examination of vital organs was carried out soon after sacrifice. Liver and kidneys were surgically removed, weighed (absolute organ weight) on an analytical balance, and immediately forwarded to the histopathological processing. The relative organ weight (ROW) of each animal was then calculated as follows: relative organ weight (%) = (organ weight/body weight) × 100 [22 (link)]. The fragments of livers and kidneys were fixed in a 10% buffered formalin solution, included in paraffin wax, cut into 3-4 μm sections, and stained with hematoxylin and eosin. The histological sections were then visualized under optical microscopy (Optika Microscopes, Italy) and captured by an Infinity 1 camera microscope with ×40 magnification.
Histopathological analysis consists of the observation of tissue integrity, searching for injuries such as degeneration, necrosis, apoptosis, and infiltration of leukocytes which could indicate signs of toxicity.