Thymuses, lymph nodes and spleens were homogenized and washed in RPMI medium containing 10% (vol/vol) FCS. Splenocytes were depleted from erythrocytes using the Red Blood Cell Lysis buffer 1x (Biolegend).
Red blood cell lysis buffer 1x
Manufactured by BioLegend
Red Blood Cell Lysis buffer 1x is a solution used to selectively lyse (break down) red blood cells in a sample, while preserving other cell types such as white blood cells. This allows for the enrichment and analysis of non-red blood cell populations.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using red blood cell lysis buffer 1x
1
Isolation of Immune Cell Populations
2
Isolation of Immune Cell Populations
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!